Actin is a Constituent of the Nuclear Matrix and Chromosome Scaffold in Physarum poly  cephalum

Abstract

Abstract: Nuclear matrices and chromosome scaffolds were obtained by digesting and extracting, respectively with DNase Ⅰ and 2 mol/L NaC1, the nuclei and chromosomes isolated from the plasmedia of Physarum polycephalum Schw. The results of the indirect immunofluorescence of tacit antiactin antibody as immunomarker indicated that the nuclear matrices and chromosome scaffolds both had positive reaction with the antibody. The results of the indirect immunodotting experiment further verified the presence of actin antibody in their constituent. Protein A-colloidal gold immunoelectron microscopy technique revealed that gold particles were distributed in the interphase nuclei and metaphase chromosomes. The above results showed that actin is a constituent of the nuclear matrix and chromosome scaffold of P. polycephalum.

Key words: Physarum polycephalum, Actin, Nuclear matrix, Chromosome scaffold

Zeng Xian-lu, Jiao Ming-da, Wang Xiao-guang and Hao Shui. Actin is a Constituent of the Nuclear Matrix and Chromosome Scaffold in Physarum poly cephalum[J]. J Integr Plant Biol., 1997, 39(8): -.

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Actin is a Constituent of the Nuclear Matrix and Chromosome Scaffold in Physarum poly cephalum

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