J Integr Plant Biol. ›› 1997, Vol. 39 ›› Issue (1): -.

• Research Articles •    

Characteristics of ABA Binding Proteins on the Plasmalemma of Guard Cells in Vicia faba

Wang Heng-bin, Wang Xue-chen, Chen Jia, Cao Min and Li Yun-yin   

Abstract: Using Viciafaba L. as the experimental material, the authors had demonstrated a kind of binding protein at the outside of plasmalemma which was highly affined to ABA. The optimum pH for the binding was 6.5; the binding activity at 25 ℃ was relatively higher than that at 0 ℃ and reaching its maximum after 30 minutes incubation. The dissociation constant of the ABA binding to the protein was 2.0 Ï 10-8 mol/L. There were about 3.2 Ï 106 binding sites per guard cell protoplast(GCP). There was functionally essential disulfide bond in the protein and the function of the protein was related to the existence of Ca2 + in the medium.

Key words: Vicia faba, Guard cell protoplasts, ABA binding proteins

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q