J Integr Plant Biol. ›› 1990, Vol. 32 ›› Issue (10): -.

• Research Articles •    

Gene Transfer into Intact Plant Cells by Electroporation

Xu Hua-qiang,Cai Guo-ping,Wu Yi,Zhao Nan-ming, Tang Hui-xian. Fu You-ying,Jiang Shuang-ying, Ren Yan-guo and Jia Shi-rong   

Abstract: The effects of electric field pulses on the permeability and viability of intact suspension cells of Chinese cabbage were investigated. Intact ceils were found to be more resistant to the detrimental effects of electric field than that of the protoplasts. Pretreatment of intact cells by heat shock (45℃, 10 min), acid medium (pH 4.0) or β-mercaptoethanol considerably increased thc transfer efficiency. CAT (chloramphenlcol acetyltransferase) activity was detected in the intact suspension cells of cucumber and rice electroporated with CAT gene.

Key words: lntact plant cells, Electroporation, Chinese cabbage, Cucumber, Rice, Wheat

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