J Integr Plant Biol. ›› 1990, Vol. 32 ›› Issue (10): -.

• Research Articles •    

Hormonal Regulation and Effect of L-Proline on Somatic Embryogenesis of Sainfoin

Lu Tie-gang, Sun ,Jing-san and Zheng Guo-chang   

Abstract: The effects of BA and KT on somatic embryogenesis of sainfoin (Onobrychis viciaefolia Scop) have been studied. Light-yellow callus was able to be formed from sainfoin hypocotyl explants cultured on LS medium containing I mg/l BA+ 1 mg/1 KT, and its somatic embryogenesis was induced by 1 mg/l BA and inhibited by 1 rog/1 KT on LS medium. Analysis of free amino acid in different types of calli indicated that the content of L-proline in embryonic callus was only as much as 2/5 of that in nonembryonic callus. Addition of L-proline to culture medium led to the stimulative effects on somatic embryogenesis in sainfoin and its optimal concentration is 1000 mg/1.

Key words: Onobrychis viciaefolia Scop., Somatic embryogenesis, L-proline

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