J Integr Plant Biol. ›› 1988, Vol. 30 ›› Issue (4): -.

• Research Articles •    

The Transference of Ri-Plasmid from Agrobacterium rhizogenes to Scopolia lurida Revealed by the Hairy Root Cultivation and the Alkaloids Production

Zhang Yin-lin   

Abstract: The steriled plants of Scopolia lurida after being infected by A. rhizogenes were induced to produce hairy roots. They grew well in medium either of hormone free MS agar or of roots suspension culture. As the result, the cultivated hairy root was shown to yield alkaloids such as hyosciamine and scopolamine. Since agropine and mannopine were also detected in the hairy root cultures, the transference of the T-DNA of A. rhizogenes Ri-plasmid into the cell genomic DNA of S. lurida is clearly demonstrated.

Key words: Transference of Ri-plasmid, Hairy root, Alkaloids

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