J Integr Plant Biol. ›› 1987, Vol. 29 ›› Issue (6): -.

• Research Articles •    

Selection of Lysine Plus Threonine-Resistant Mutant of Maize

Miao Shu-hua, D. R. Duncan, and J. M. Widholm   

Abstract: Resistance to certain amino acids or amino acid analogs can lead to overproduce specific 'free amino acids. By selection-Mutagenic treatment-Selection, lysine plus threonine-resistant mutant (RLT) was obtained from tissue culture of maize, W77-R3019V The resistance of RLT was 20 times higher than that of wild type. The levels of all free aspartate family amino acids in RLT were higher than those in wild type. Especially, threonine was 20 times higher. The resistance was inheritable and segregation in progenies, RLT1 and F1, was approximate to 3:1 and 1:1 resistant/sensitive ratio, respectively. The resistance was inherited as a single dominant or semidominant nuclear gene. In RLT2 embryo cultures, the resistance and free threonine levels in resistant callus were 20 and 23 times higher than those in sensitive one, respectively. In the homozygous seeds of RLT2, the levels of free threonine, arginine, lysine, methionine and isoleucine were 11, 8, 5, 5 and 3 times higher than those of wild type.

Key words: Maize, Tissue culture, Lysine plus threonine resistance, Inheritance and expression

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