J Integr Plant Biol. ›› 1987, Vol. 29 ›› Issue (3): -.

• Research Articles •    

Effect of Auxin, Kinetin and Monosaccharide on Callus Cell wall Composition of Abutilon avicennae Gaertn

Wei Yu-ning and Li Yao-dong   

Abstract: The effects of exogenous kinetin, IAA, glucose (the component of cellulose) and galactose (one of tile inhibiting compounds of cellulose synthesis) on callus cell wall composition of China jute (Abutilon avicennae Gaertn) were investigated. When the China jute callus materials were treated with 2 mg/l IAA and H3-glucose, there was a 437% increase of the Ha-glucose, as compared with control. When galactose was added simultaneously with H3-glucose, there was a 138% increase of the H3-glucose as compared with control. Galactose inhibited H3-glucose incorporate content in cellulose, and also in pectin and hemicellulose. In the experiment with 10 mg/l kinetin treatment of the callus materials, when galactose was added, the incorporation H3-glucose was inhibited. It is interesting that the kinetin enhanced H3-galactose incorporation into all cell wall components. When it was added simultaneously with glucose, glucose inhibited H3-galactose into the cell Wall components. Results showed that the hormones effect cell wall component, not only concerned with hormones, but also concerned with exogenous monosaccharides. When exogenous monosacchrides were added to the culture medium (as galactose, glucose) in combination or respectively, they are quite different in effecting hormone induced China jute callus cell wall. When the callus was treated by IAA, galactose inhibited H3-glucose incorporation into cell wall components. When it was treated by kinetin, glucose inhibited H3-galactose incorporation into cell wall components.

Key words: Callus, Exogenous monosaccharide, Cell wall composition

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