J Integr Plant Biol. ›› 2016, Vol. 58 ›› Issue (7): 618-622.DOI: 10.1111/jipb.12457

• Letters to the Editor • Previous Articles     Next Articles

Depletion of tyrosyl-DNA phosphodiesterase 1α (MtTdp1α) affects transposon expression in Medicago truncatula

Maria Elisa Sabatini1, Mattia Donà1†, Paola Leonetti2, Andrea Minio3, Massimo Delledonne3, Daniela Carbonera1, Massimo Confalonieri4, Giorgio Giraffa4 and Alma Balestrazzi1*   

  1. 1Department of Biology and Biotechnology ‘L. Spallanzani‘ 27100 Pavia, Italy
    2Institute for Sustainable Plant Protection, National Research Council (CNR), Bari, Italy
    3Department of Biotechnology, University of Verona, Verona, Italy
    4Fodder and Dairy Production Research Centre, Lodi, Italy
  • Received:2015-04-20 Accepted:2015-12-18 Published:2015-12-23
  • About author:Present address: Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Dr. Bohr Gasse 3, 1030 Vienna, Austria
    *Correspondence: E-mail: alma.balestrazzi@unipv.it

Abstract:

The role of plant tyrosyl-DNA phosphodiesterase 1α in genome stability is studied using a Medicago truncatula MtTdp1α-depleted line. Lack of MtTdp1α results in a 39% reduction of methylated cytosines as compared to control. RNA-Seq analyses revealed that 11 DNA transposons and 22 retrotransposons were differentially expressed in the Tdp1α-2a line. Among them all, DNA transposons (MuDR, hAT, DNA3-11_Mad) and seven retrotransposons (LTR (Long Terminal Repeat)/Gipsy, LTR/Copia, LTR and NonLTR/L1) were down-regulated, while the 15 retrotransposons were up-regulated. Results suggest that the occurrence of stress-responsive cis-elements as well as changes in the methylation pattern at the LTR promoters might be responsible for the enhanced retrotransposon transcription.

Key words: DNA methylation, Medicago truncatula, RNA-Seq, transposable elements, tyrosyl-DNA phosphodiesterase

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22