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J Integr Plant Biol, 1994, 36 (Suppl.): -, Research Article
Ploidy on Anther Plantlets of
Cao Zi-yi
Anthers of grape cultivar “Pobeda” (Vitis vinefera) were inoculated on the same medium for induction and differentiation and cultured in the same conditions, and embryogenic calli from anthers were induced, then regenerated plantlets were obtained. Finally they survived through transplanting. The frequency of induction and differentiation, however, had some changes in different ecogeographic conditions and gathered times of anthers, All of regenerated plantlets were diploid by identification chromosomes in root tip cells. Author did not find any typical polycetl of poly-nucleus pollens determined during culture. It showed that all induced plantlets from anthers in these ecogeographic conditions come from anther sometic cells and not from pollen. Anther culture of cultivar “Pobeda” could not obtained regenerated haploid plantlets through testing in given regions for many years.
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