J Integr Plant Biol. ›› 2015, Vol. 57 ›› Issue (12): 1031-1045.DOI: 10.1111/jipb.12328

• Functional Omics and Systems Biology • Previous Articles     Next Articles

Differential transcriptome analysis between Populus and its synthesized allotriploids driven by second-division restitution

Shiping Cheng1,2, Zhen Huang1,2, Yun Li1,2, Ting Liao1,2, Yujing Suo1,2, Pingdong Zhang1,2, Jun Wang1,2 and Xiangyang Kang1,2*   

  1. 1National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
    2Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
  • Received:2014-09-28 Accepted:2014-12-23 Published:2014-12-30
  • About author:*Correspondence: E-mail: kangxy@bjfu.edu.cn

Abstract:

In this report, we compared transcriptomic differences between a synthetic Populus section Tacamahaca triploid driven by second-division restitution and its parents using a high-throughput RNA-seq method. A total of 4,080 genes were differentially expressed between the high-growth vigor allotriploids (SDR-H) and their parents, and 719 genes were non-additively expressed in SDR-H. Differences in gene expression between the allotriploid and male parent were more significant than those between the allotriploid and female parent, which may be caused by maternal effects. We observed 3,559 differentially expressed genes (DEGs) between the SDR-H and male parent. Notably, the genes were mainly involved in metabolic process, cell proliferation, DNA methylation, cell division, and meristem and developmental growth. Among the 1,056 DEGs between SDR-H and female parent, many genes were associated with metabolic process and carbon utilization. In addition, 1,789 DEGs between high- and low-growth vigor allotriploid were mainly associated with metabolic process, auxin poplar transport, and regulation of meristem growth. Our results indicated that the higher poplar ploidy level can generate extensive transcriptomic diversity compared with its parents. Overall, these results increased our understanding of the driving force for phenotypic variation and adaptation in allopolyploids driven by second-division restitution.

 

Cheng S, Huang Z, Li Y, Liao T, Suo Y, Zhang P, Wang J, Kang X (2015) Differential transcriptome analysis between Populus and its synthesized allotriploids driven by second-division restitution. J Integr Plant Biol 57: 1031–1045 doi: 10.1111/jipb.12328

Key words: High-growth vigor, poplar allotriploid, RNA-seq, second-division restitution, transcriptome

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2021 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22