J Integr Plant Biol. ›› 2021, Vol. 63 ›› Issue (4): 737-755.DOI: 10.1111/jipb.13043

Special Issue: Development Hormone signaling

• Molecular Physiology • Previous Articles     Next Articles

AtNSF regulates leaf serration by modulating intracellular trafficking of PIN1 in Arabidopsis thaliana

Li Ping Tang1,2†, Yi Yang1,2†, Hui Wang3, Lixin Li4, Le Liu3, Yu Liu1,2, Jinfeng Yuan1,2, Xiang Yu Zhao1,2, Klaus Palme2,3,5,6, Ying Hua Su1,2* and Xugang Li1,2*   

  1. 1State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China
    2Sino‐German Joint Research Center on Agricultural Biology, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China
    3Institute of Biology II/Molecular Plant Physiology, Faculty of Biology, Albert‐Ludwigs‐University of Freiburg, Schänzlestrasse 1, Freiburg D‐79104, Germany
    4Key Laboratory of Saline‐alkali Vegetation Ecology Restoration, Ministry of Education, Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Harbin 150040, China
    5BIOSS Centre for Biological Signalling Studies, Albert‐Ludwigs‐University Freiburg, Signalhaus, Schänzlestr. 18, Freiburg D‐79104, Germany
    6Center for Biological Systems Analysis (ZBSA), Albert‐Ludwigs‐University Freiburg, Freiburg D‐79104, Germany

    These authors contributed equally to this work.
    *Correspondences: Xugang Li (xgli@sdau.edu.cn, Dr. Li is responsible for the distributions of all materials associated with this article); Ying Hua Su (suyh@sdau.edu.cn)
  • Received:2020-08-18 Accepted:2020-11-30 Online:2020-12-02 Published:2021-04-01

Abstract: In eukaryotes, N‐ethylmaleimide‐sensitive factor (NSF) is a conserved AAA+ATPase and a key component of the membrane trafficking machinery that promotes the fusion of secretory vesicles with target membranes. Here, we demonstrate that the Arabidopsis thaliana genome contains a single copy of NSF, AtNSF, which plays an essential role in the regulation of leaf serration. The AtNSF knock‐down mutant, atnsf‐1, exhibited more serrations in the leaf margin. Moreover, polar localization of the PIN‐FORMED1 (PIN1) auxin efflux transporter was diffuse around the margins of atnsf‐1 leaves and root growth was inhibited in the atnsf‐1 mutant. More PIN1‐GFP accumulated in the intracellular compartments of atnsf‐1 plants, suggesting that AtNSF is required for intracellular trafficking of PIN between the endosome and plasma membrane. Furthermore, the serration phenotype was suppressed in the atnsf‐1 pin1‐8 double mutant, suggesting that AtNSF is required for PIN1‐mediated polar auxin transport to regulate leaf serration. The CUP‐SHAPED COTYLEDON2 (CUC2) transcription factor gene is up‐regulated in atnsf‐1 plants and the cuc2‐3 single mutant exhibits smooth leaf margins, demonstrating that AtNSF also functions in the CUC2 pathway. Our results reveal that AtNSF regulates the PIN1‐generated auxin maxima with a CUC2‐mediated feedback loop to control leaf serration.

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