J Integr Plant Biol. ›› 2021, Vol. 63 ›› Issue (7): 1309-1323.DOI: 10.1111/jipb.13085

Special Issue: Evolution

• Functional Omics and Systems Biology • Previous Articles     Next Articles

Insights into salvianolic acid B biosynthesis from chromosome-scale assembly of the Salvia bowleyana genome

Xuehai Zheng, Duo Chen, Binghua Chen, Limin Liang, Zhen Huang, Wenfang Fan, Jiannan Chen, Wenjin He, Huibin Chen, Luqiang Huang, Youqiang Chen, Jinmao Zhu and Ting Xue*   

  1. The Public Service Platform for Industrialization Development Technology of Marine Biological Medicine and Products of the State Oceanic Administration, Fujian Key Laboratory of Special Marine Bioresource Sustainable Utilization, Southern Institute of Oceanography, College of Life Sciences, Fujian Normal University, Fuzhou 350117, China

    These authors contributed equally to this work.
    *Correspondence: Ting Xue (xueting@fjnu.edu.cn)
  • Received:2021-01-11 Accepted:2021-02-26 Online:2021-02-26 Published:2021-07-01

Abstract: Salvia bowleyana is a traditional Chinese medicinal plant that is a source of nutritional supplements rich in salvianolic acid B and a potential experimental system for the exploration of salvianolic acid B biosynthesis in the Labiatae. Here, we report a high-quality chromosome-scale genome assembly of S. bowleyana covering 462.44 Mb, with a scaffold N50 value of 57.96 Mb and 44,044 annotated protein-coding genes. Evolutionary analysis revealed an estimated divergence time between S. bowleyana and its close relative S. miltiorrhiza of ~3.94 million years. We also observed evidence of a whole-genome duplication in the S. bowleyana genome. Transcriptome analysis showed that SbPAL1 (PHENYLALANINE AMMONIA-LYASE1) is highly expressed in roots relative to stem and leaves, paralleling the location of salvianolic acid B accumulation. The laccase gene family in S. bowleyana outnumbered their counterparts in both S. miltiorrhiza and Arabidopsis thaliana, suggesting that the gene family has undergone expansion in S. bowleyana. Several laccase genes were also highly expressed in roots, where their encoded proteins may catalyze the oxidative reaction from rosmarinic acid to salvianolic acid B. These findings provide an invaluable genomic resource for understanding salvianolic acid B biosynthesis and its regulation, and will be useful for exploring the evolution of the Labiatae.

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