J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (4): 934-949.DOI: 10.1111/jipb.13429

• Abiotic Stress Responses • Previous Articles     Next Articles

ART1 and putrescine contribute to rice aluminum resistance via OsMYB30 in cell wall modification

Li Jun Gao1,2, Xiang Pei Liu3, Ke Ke Gao1, Meng Qi Cui1, Hui Hui Zhu1, Gui Xin Li4, Jing Ying Yan5, Yun Rong Wu1, Zhong Jie Ding1, Xue Wei Chen6, Jian Feng Ma7, Nicholas P. Harberd8 and Shao Jian Zheng1,2*   

  1. 1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China;
    2. Guangdong Laboratory for Lingnan Modern Agriculture, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 5100642, China;
    3. Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China;
    4. College of Agronomy and Biotechnology, Zhejiang University, Hangzhou 310058, China;
    5. Agricultural Experimental Station, Zhejiang University, Hangzhou 310058, China;
    6. Rice Research Institute, Sichuan Agricultural University at Wenjiang, Chengdu 611130, China;
    7. Institute of Plant Science and Resources, Okayama University, Kurashiki 710‐0046, Japan;
    8. Department of Plant Science, University of Oxford, Oxford OX1 3RB, United Kingdom
    *Correspondence: Shao Jian Zheng (sjzheng@zju.edu.cn)
  • Received:2022-08-15 Accepted:2022-12-09 Online:2022-12-14 Published:2023-04-01

Abstract: Cell wall is the first physical barrier to aluminum (Al) toxicity. Modification of cell wall properties to change its binding capacity to Al is one of the major strategies for plant Al resistance; nevertheless, how it is regulated in rice remains largely unknown. In this study, we show that exogenous application of putrescines (Put) could significantly restore the Al resistance of art1, a rice mutant lacking the central regulator Al RESISTANCE TRANSCRIPTION FACTOR 1 (ART1), and reduce its Al accumulation particularly in the cell wall of root tips. Based on RNA‐sequencing, yeast‐one‐ hybrid and electrophoresis mobility shift assays, we identified an R2R3 MYB transcription factor OsMYB30 as the novel target in both ART1‐ dependent and Put‐promoted Al resistance. Furthermore, transient dual‐luciferase assay showed that ART1 directly inhibited the expression of OsMYB30, and in turn repressed Os4CL5‐ dependent 4‐coumaric acid accumulation, hence reducing the Al‐binding capacity of cell wall and enhancing Al resistance. Additionally, Put repressed OsMYB30 expression by eliminating Al‐ induced H2O2 accumulation, while exogenous H2O2 promoted OsMYB30 expression. We concluded that ART1 confers Put‐promoted Al resistance via repression of OsMYB30‐regulated modification of cell wall properties in rice.

Key words: ART1, cell wall, 4‐coumaric acid, OsMYB30, Os4CL5, putrescine

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