J Integr Plant Biol. ›› 2015, Vol. 57 ›› Issue (10): 830-837.DOI: 10.1111/jipb.12312

• Molecular Physiology • Previous Articles     Next Articles

Glucose alleviates cadmium toxicity by increasing cadmium fixation in root cell wall and sequestration into vacuole in Arabidopsis

Yuan-Zhi Shi1,2†, Xiao-Fang Zhu3†, Jiang-Xue Wan2, Gui-Xin Li4 and Shao-Jian Zheng2*   

  1. 1Tea Research Institute of the Chinese Academy of Agricultural Sciences and the Key Laboratory for Plant Biology and Resource Application of Tea, the Ministry of Agriculture, Hangzhou, China
    2State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China
    3State Key Laboratory of Soil and Sustainable Agriculture, China Institute of Soil Science, the Chinese Academy of Science, Nanjing, China
    4College of Agronomy and Biotechnology, Zhejiang University, Hangzhou, China
  • Received:2014-07-30 Accepted:2014-11-17 Published:2014-11-17
  • About author:These authors contributed equally to this work.
    *Correspondence: E-mail: sjzheng@zju.edu.cn

Abstract:

Glucose (Glu) is involved in not only plant physiological and developmental events but also plant responses to abiotic stresses. Here, we found that the exogenous Glu improved root and shoot growth, reduced shoot cadmium (Cd) concentration, and rescued Cd-induced chlorosis in Arabidopsis thaliana (Columbia ecotype, Col-0) under Cd stressed conditions. Glucose increased Cd retained in the roots, thus reducing its translocation from root to shoot significantly. The most Cd retained in the roots was found in the hemicellulose 1. Glucose combined with Cd (Glu + Cd) treatment did not affect the content of pectin and its binding capacity of Cd while it increased the content of hemicelluloses 1 and the amount of Cd retained in it significantly. Furthermore, Leadmium Green staining indicated that more Cd was compartmented into vacuoles in Glu + Cd treatment compared with Cd treatment alone, which was in accordance with the significant upregulation of the expression of tonoplast-localized metal transporter genes, suggesting that compartmentation of Cd into vacuoles also contributes to the Glu-alleviated Cd toxicity. Taken together, we demonstrated that Glu-alleviated Cd toxicity is mediated through increasing Cd fixation in the root cell wall and sequestration into the vacuoles.

 

Shi YZ, Zhu XF, Wan JX, Li GX, Zheng SJ (2015) Glucose alleviates cadmium toxicity by increasing cadmium fixation in root cell wall and sequestration into vacuole in Arabidopsis. J Integr Plant Biol 57: 830–837 doi: 10.1111/jipb.12312

Key words: Arabidopsis, cadmium tolerance, glucose, plant cell wall, plant vacuole

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2021 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22