Author: Cong Zhou, Qian Zhang, Yu Chen, Jin Huang, Qin Guo, Yi Li, Wensheng Wang, Yongfu Qiu, Wei Guan, Jing Zhang, Jianping Guo, Shaojie Shi, Di Wu, Xiaohong Zheng, Lingyun Nie, Jiaoyan Tan, Chaomei Huang, Yinhua Ma, Fang Yang, Xiqin Fu, Bo Du, Lili Zhu, Rongzhi Chen, Zhikang Li, Longping Yuan and Guangcun He
J Integr Plant Biol 2021, 63 (10): 1695-1711.
Interactions and co-evolution between plants and herbivorous insects are critically important in agriculture. Brown planthopper (BPH) is the most severe insect of rice, and the biotypes adapt to feed on different rice genotypes. Here, we present genomics analyses on 1,520 global rice germplasms for resistance to three BPH biotypes. Genome-wide association studies identified 3,502 single nucleotide polymorphisms (SNPs) and 59 loci associated with BPH resistance in rice. We cloned a previously unidentified gene
Bph37 that confers resistance to BPH. The associated loci showed high nucleotide diversity. Genome-wide scans for trans-species polymorphisms revealed ancient balancing selection at the loci. The secondarily evolved insect biotypes II and III exhibited significantly higher virulence and overcame more rice varieties than the primary biotype I. In response, more SNPs and loci evolved in rice for resistance to biotypes II and III. Notably, three exceptional large regions with high SNP density and resistance-associated loci on chromosomes 4 and 6 appear distinct between the resistant and susceptible rice varieties. Surprisingly, these regions in resistant rice might have been retained from wild species Oryza nivara. Our findings expand the understanding of long-term interactions between rice and BPH and provide resistance genes and germplasm resources for breeding durable BPH-resistant rice varieties.