Please wait a minute...
J Integr Plant Biol, 2005, 47 (1): 50-59, Research Article
Purification, Biochemical and Immunological Characterization of Acid Invertases from Apple Fruit
Qiu-Hong PAN, Ke-Qin ZOU, Chang-Cao PENG, Xiu-Ling WANG and Da-Peng ZHANG
doi: 10.1111/j.1744-7909.2005.00018.x
Abstract
The soluble acid invertase (SAI) and cell wall-bound invertase (CWI) were purified from apple fruit to apparent electrophoretic homogeneity. Based on sequencing, substrate specificity, and immunoblotting assay, the purified enzymes were identified to be two isoforms of acid invertase (b-fructosidase; EC 3.2.1.26). The SAI and CWI have the same apparent molecular mass with a holoenzyme of molecular mass of 220 kDa composed of 50 kDa subunits. The SAI has a lower Km value for sucrose and higher Km for raffinose compared with CWI. These acid invertases differ from those in other plants in some of their biochemical properties, such as the extremely high Km value for raffinose, no hydrolytic activity for stachyose, and a mixed form of inhibition by fructose to their activity. The antibodies directed against the SAI and CWI recognized, from the crude extract, three polypeptides with a molecular mass of 50, 68, and 30 kDa, respectively. These results provide a substantial basis for the further studies of the acid invertases in apple fruit.
--------------------------------------------------------------------------------------------------------------------
   Online on:
This Article
   Full Text  
   Full Text (PDF) 
   Supporting information
Services
   E-mail Link to this Article
   Export Citation for this Article
Citing Articles
   Cited By
Google Scholar
   Articles by Qiu-Hong PAN
   Articles by Ke-Qin ZOU
   Articles by Chang-Cao PENG
   Articles by Xiu-Ling WANG
   Articles by Da-Peng ZHANG
PubMed
   Articles by Qiu-Hong PAN
   Articles by Ke-Qin ZOU
   Articles by Chang-Cao PENG
   Articles by Xiu-Ling WANG
   Articles by Da-Peng ZHANG
Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2020 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
网站备案号:京ICP备16067583号-22