J Integr Plant Biol. ›› 2005, Vol. 47 ›› Issue (5): -.DOI: 10.1111/j.1744-7909.2005.00055.x

• Research Articles •    

Identification and Characterization of Reverse Transcriptase Domain of Transcriptionally Active Retrotransposons in Wheat Genomes

Yi-Miao TANG, You-Zhi MA, Lian-Cheng LI ,Xing-Guo YE   

Abstract: To clarify activation characterization of wheat (Triticum aestivum L.) retrotransposons, transcriptionally active Ty1-copia retrotransposons were found in wheat by using RT-PCR to amplify the RT domain. Sequence analysis of random RT-PCR clones reveals that Ty1-copia retrotransposons are highly heterogeneous and can be divided into at least four groups, which are tentatively named TaRT-1 to TaRT-4. Dot blot hybridization indicates that TaRT-1 exists in the wheat genome as multiple copies (at 30 000 copies/a hexaploid genome (ABD)). Northern blot hybridization showed that TaRT-1 is only expressed at a low level under normal conditions in seedlings, but at a high level when induced by powdery mildew fungus, jasmonic acid (JA) and salicylic acid (SA). These results suggest that the TaRT-1 expression is highly sensitive to biotic and abiotic stresses.

Key words: abiotic stress, biotic stress, retrotransposons, reverse transcriptase, Triticum, wheat.

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