J Integr Plant Biol. ›› 2015, Vol. 57 ›› Issue (7): 628-640.DOI: 10.1111/jipb.12302

• Plant-environmental Interactions • Previous Articles     Next Articles

Hydrogen sulfide regulates abiotic stress tolerance and biotic stress resistance in Arabidopsis

Haitao Shi1, Tiantian Ye1,2, Ning Han3, Hongwu Bian3, Xiaodong Liu4 and Zhulong Chan1*   

  1. 1Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, the Chinese Academy of Sciences, Wuhan, China
    2University of Chinese Academy of Sciences, Beijing, China
    3Institute of Genetics, State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China
    4College of Agronomy, Xinjiang Agricultural University, Urumqi, China
  • Received:2014-08-26 Accepted:2014-10-17 Published:2014-10-20
  • About author:*Correspondence: E-mail: zhulongch@wbgcas.cn

Abstract:

Hydrogen sulfide (H2S) is an important gaseous molecule in various plant developmental processes and plant stress responses. In this study, the transgenic Arabidopsis thaliana plants with modulated expressions of two cysteine desulfhydrases, and exogenous H2S donor (sodium hydrosulfide, NaHS) and H2S scavenger (hypotaurine, HT) pre-treated plants were used to dissect the involvement of H2S in plant stress responses. The cysteine desulfhydrases overexpressing plants and NaHS pre-treated plants exhibited higher endogenous H2S level and improved abiotic stress tolerance and biotic stress resistance, while cysteine desulfhydrases knockdown plants and HT pre-treated plants displayed lower endogenous H2S level and decreased stress resistance. Moreover, H2S upregulated the transcripts of multiple abiotic and biotic stress-related genes, and inhibited reactive oxygen species (ROS) accumulation. Interestingly, MIR393-mediated auxin signaling including MIR393a/b and their target genes (TIR1, AFB1, AFB2, and AFB3) was transcriptionally regulated by H2S, and was related with H2S-induced antibacterial resistance. Moreover, H2S regulated 50 carbon metabolites including amino acids, organic acids, sugars, sugar alcohols, and aromatic amines. Taken together, these results indicated that cysteine desulfhydrase and H2S conferred abiotic stress tolerance and biotic stress resistance, via affecting the stress-related gene expressions, ROS metabolism, metabolic homeostasis, and MIR393-targeted auxin receptors.

 

Shi H, Ye T, Han N, Bian H, Liu X, Chan Z (2015) Hydrogen sulfide regulates abiotic stress tolerance and biotic stress resistance in Arabidopsis. J Integr Plant Biol 57: 628–640. doi: 10.1111/jipb.12302

Key words: Abiotic stress, auxin receptor, biotic stress, cysteine desulfhydrase, hydrogen sulfide, MIR393

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