J Integr Plant Biol ›› 2015, Vol. 57 ›› Issue (5): 450-467.DOI: 10.1111/jipb.12288

Special Issue: Cell Biology and Functions

• Cell and Developmental Biology •     Next Articles

Constructing a high-density linkage map for Gossypium hirsutum × Gossypium barbadense and identifying QTLs for lint percentage

Yuzhen Shi1†, Wentan Li1,2†, Aiguo Li1,3†, Ruihua Ge1,4, Baocai Zhang1, Junzhi Li1,4, Guangping Liu1,4, Junwen Li1, Aiying Liu1, Haihong Shang1, Juwu Gong1, Wankui Gong1, Zemao Yang1,4, Feiyü Tang2, Zhi Liu4, Weiping Zhu4, Jianxiong Jiang4, Xiaonan Yu5, Tao Wang1, Wei Wang1, Tingting Chen1, Kunbo Wang1, Zhengsheng Zhang6 and Youlu Yuan1*   

  1. 1State Key Laboratory of Cotton Biology, Key Laboratory of Biological and Genetic Breeding of Cotton, the Ministry of Agriculture, Institute of Cotton Research, the Chinese Academy of Agricultural Sciences, Anyang, China
    2Agricultural College of Jiangxi Agricultural University, Nanchang, China
    3Academy of Agricultural Sciences of Luohe City of Henan Province, Luohe, China
    4College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China
    5College of Agronomy, Hunan Agricultural University, Changsha, China
    6Agricultural College of Southwest University, Chongqing, China
  • Received:2014-06-18 Accepted:2014-09-21 Published:2014-09-27
  • About author:These authors contributed equally to this work.
    *Correspondence: E-mail: youluyuan@hotmail.com

Abstract:

To introgress the good fiber quality and yield from Gossypium barbadense into a commercial Upland cotton variety, a high-density simple sequence repeat (SSR) genetic linkage map was developed from a BC1F1 population of Gossypium hirsutum × Gossypium barbadense. The map comprised 2,292 loci and covered 5115.16 centiMorgan (cM) of the cotton AD genome, with an average marker interval of 2.23 cM. Of the marker order for 1,577 common loci on this new map, 90.36% agrees well with the marker order on the D genome sequence genetic map. Compared with five published high-density SSR genetic maps, 53.14% of marker loci were newly discovered in this map. Twenty-six quantitative trait loci (QTLs) for lint percentage (LP) were identified on nine chromosomes. Nine stable or common QTLs could be used for marker-assisted selection. Fifty percent of the QTLs were from G. barbadense and increased LP by 1.07%–2.41%. These results indicated that the map could be used for screening chromosome substitution segments from G. barbadense in the Upland cotton background, identifying QTLs or genes from G. barbadense, and further developing the gene pyramiding effect for improving fiber yield and quality.

 

Shi Y, Li W, Li A, Ge R, Zhang B, Li J, Liu G, Li J, Liu A, Shang H, Gong J, Gong W, Yang Z, Tang F, Liu Z, Zhu W, Jiang J, Yu X, Wang T, Wang W, Chen T, Wang K, Zhang Z, Yuan Y (2015) Constructing a high‐density linkage map for Gossypium hirsutum × Gossypium barbadense and identifying QTLs for lint percentage. J Integr Plant Biol 57: 450–467. doi: 10.1111/jipb.12288

Upland cotton has high yield and Island cotton has superior fiber properties. To introgress the good fiber quality from Island cotton into an Upland cotton variety, a high-density genetic linkage map, with 2,292 loci and covered 5,115.16cM of the cotton genome, was developed from an interspecific population.

Key words: Cotton (Gossypium spp.), genetic linkage map, Gossypium barbadense, lint percentage, simple sequence repeat

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