J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (2): 155-166.DOI: 10.1111/j.1744-7909.2008.00782.x

Special Issue: Cell Biology and Functions

• Cell and Developmental Biology • Previous Articles     Next Articles

Identification and expression of floral organ homeotic genes from Alpinia oblongifolia (Zingiberaceae)

Yong-Mei Xia, Xue-Mei Gao and Qing-Jun Li   

  • Received:2008-04-23 Accepted:2008-08-31 Published:2009-02-10

Abstract:   Current understanding of the classical ABC model of floral development has provided a new set of characters to evaluate floral evolution. However, what is still lacking is a clear assessment of this genetic program across monocots. Here, to investigate the evolution of members of class A and B genes in monocots, we report the sequence characteristic and transcript expression of three new MADS‐box genes in Alpinia oblongifolia Hayata. Sequence and phylogenetic analysis reveals that these genes are FUL‐like and AP3‐like. Therefore, they were termed AoFL1, AoFL2 and AoAP3. AoFL1 contains the FUL motif, but AoFL2 lacks this motif. Their expression revealed by in situ hybridization may reflect the ancestral function of FUL‐like genes in the specification of inflorescence and floral meristems. The AoAP3 gene contains two conserved motifs, the PI‐derived and paleoAP3 motifs. The AoAP3 transcripts located to the corolla and stamen, and hybridization signals were detected in the central whorl. These expression patterns suggest that the functions of homologous organ identity genes are diversified in A. oblongifolia. The implications of these findings on the conservation of homologous gene function are discussed.

Key words: MADS-box genes, ABC model, Floral organ identity, Phylogenetic analysis, In situ hybridization

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2021 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22