J Integr Plant Biol. ›› 2008, Vol. 50 ›› Issue (2): 183-189.DOI: 10.1111/j.1744-7909.2007.00588.x

• Development & Photosynthesis • Previous Articles     Next Articles

Characters of DNA Constitution in the Rye B Chromosome

Hong Long, Zhong-Xia Qi, Xiao-Ming Sun, Cheng-Bin Chen, Xiu-Lan Li, Wen-Qin Song and Rui-Yang Chen   

Abstract: We have used chromosome microdissection and microcloning to construct a DNA library of the entire B chromosome (B) of rye. New rye B-specific sequences have been screened from this pool, blasted with other sequences and analyzed to elucidate the characters of DNA constitution and the possible pathway of the origin of the rye B chromosome. We report the discovery of a new sequence that is specific to the rye B centromere.

Key words: B chromosome, fluorescence in situ hybridization, microdissection, rye.

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