J Integr Plant Biol ›› 2009, Vol. 51 ›› Issue (6): 562-573.DOI: 10.1111/j.1744-7909.2009.00832.x

Special Issue: Plant Signal Transduction

• Plant-environmental Interactions • Previous Articles     Next Articles

Jasmonic Acid is Induced in a Biphasic Manner in Response of Pea Seedlings to Wounding

Hao-Ru Yang1†, Ke Tang1†, Hong-Tao Liu2, Qiu-Hong Pan1 and Wei-Dong Huang1*   

  1. 1 College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
    2 Institute of Geographic Sciences and Natural Resources Research, the Chinese Academy of Sciences, Beijing 100101, China
  • Received:2008-12-29 Accepted:2009-03-10 Published:2009-06-08
  • About author:
    The authors contributed equally to this work.
    *Author for correspondence Tel: +86 10 6273 7024; Fax: +86 10 6273 7553; E-mail: huanggwd@263.net

Abstract:

The role of jasmonic acid (JA) in plant wounding response has beendemonstrated. However, the source of JA in wound signaling remains unclear. In the present study, pea seedlings were used as material to investigate the systemic induction of JA and the activation of lipoxygenase (LOX)-dependent octadecanoid pathway upon wounding. The results showed that endogenous JA could induce two peaks in the wounded leaves and the stalks, while only one peak in the systemic leaves. LOX activity and its protein amount were also induced and the stimulation mainly occurred in the late phase, while one peak of induction was present after pretreatment with JA. Applied nordihydroguaiaretic acid (NDGA), an inhibitor of LOX activity, only inhibited the induction of JA in the late phase, and the resistance of pea was impaired. Furthermore, 13(S)-hydroperoxy-9(Z), 11(E)-octadecadienoic acid (13 (S)-H(P)ODE) was confirmed to be the main product of LOX throughout the experimental time. In addition, immunocytochemical analysis also revealed the occurrence of JA biosynthesis and transport upon wounding. These results demonstrated that wound-induced JA in wounded leaves resulted from its biosynthesis and conversion from its conjugates, while in systemic leaves resulted from its transport and biosynthesis; and proved that the LOX pathway was vital to the wound-induced defense response involved in JA biosynthesis.

Yang HR, Tang K, Liu HT, Pan QH, Huang WD (2009). Jasmonic acid is induced in a biphasic manner in response of pea seedlings to wounding. J. Integr. Plant Biol. 51(6), 562–573.

Key words: defense response, jasmonic acid, lipoxygenase, pea (Pisum sativum L.).

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2020 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
网站备案号:京ICP备16067583号-22