J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (9): 858-867.DOI: 10.1111/j.1744-7909.2009.00861.x

Special Issue: Abiotic Stresses

• Plant-environmental Interactions • Previous Articles     Next Articles

Osmopriming-Regulated Changes of Plasma Membrane Composition and Function were Inhibited by Phenylarsine Oxide in Soybean Seeds

Jiajin Zhuo1, Weixiang Wang2, Yun Lu1, Wu Sen1and Xiaofeng Wang1*   

  1. 1 Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 100083, China
    2 School of Bioengineering, Xihua University, Chengdu 610039, China
  • Received:2009-03-01 Accepted:2009-06-30 Published:2009-08-27
  • About author: *Author for correspondence Tel: +86 158 1081 5060; Fax: +86 010 6233 7855; E-mail: wxf801@sina.com

Abstract:

The aim of the present work was to investigate the effects of osmoconditioning on chilling injury in chilling-sensitive soybean (Glycine max (L.) Merr. Zhonghuang No. 22) seeds during imbibition. Low temperatures reduced the germination rate and no seed germinated at 1 ◦C. Osmoconditioning of seeds at 20 C with a polyethylene glycol-8000 (PEG8000) solution at 1.5 MPa for 72 h followed by drying back to their initial moisture content (MC) reduced their chilling sensitivity. The phenylarsine oxide (PAO), an inhibitor of protein tyrosinephosphatases, was used to investigate the possible involvement of phosphorylation-dephosphorylation of Tyr residues in the plasma membrane composition and function when seeds were osmoconditioned. The results showed the germination of osmoconditioned seeds decreased significantly when PAO was added in PEG solution after chilling treatment. PAO inhibited changes in composition of plasmamembrane phospholipids and fatty acid induced by osmocondition, indicated that tyrosine protein phosphorylation is involved in the regulatory mechanisms of osmocondition-responsive chilling in soybean seeds. Western blot result further indicated that osmocondition treatment improved the activity of plasma membrane H+-ATPase after chilling treatment, but this effect was abolished by PAO. The possible regulation mechanism by Tyr protein phosphorylation is discussed.
 

Zhuo JJ, Wang WX, Lu Y, Wu S, Wang XF (2009). Osmopriming-regulated changes of plasma membrane composition and function were inhibited by phenylarsine oxide in soybean seeds. J. Integr. Plant Biol. 51(9), 858–867.

Key words: fatty acid, membrane lipid, osmoconditioning, phenylarsine oxide, protein-tyr phosphatases, soybean seeds.

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