J Integr Plant Biol. ›› 2013, Vol. 55 ›› Issue (6): 527-536.DOI: 10.1111/jipb.12038

• Plant-environmental Interactions • Previous Articles     Next Articles

Nitric Oxide Blocks Blue Light-Induced K+ Influx by Elevating the Cytosolic Ca2+ Concentration in Vicia faba L. Guard Cells

Xiang Zhao, Yuan-Yuan Li, Hui-Li Xiao, Chang-Shui Xu and Xiao Zhang*   

  1. Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, College of Life Sciences, Henan University, Kaifeng 475004, China
  • Received:2012-11-06 Accepted:2013-01-27 Published:2013-04-12
  • About author:*Corresponding author Tel: +86 378 388 0008; Fax: +86 378 388 1387; E-mail:xzhang@henu.edu.cn


Ca2+ plays a pivotal role in nitric oxide (NO)-promoted stomatal closure. However, the function of Ca2+ in NO inhibition of blue light (BL)-induced stomatal opening remains largely unknown. Here, we analyzed the role of Ca2+ in the crosstalk between BL and NO signaling in Vicia faba L. guard cells. Extracellular Ca2+ modulated the BL-induced stomatal opening in a dose-dependent manner, and an application of 5 μM Ca2+ in the pipette solution significantly inhibited BL-activated K+ influx. Sodium nitroprusside (SNP), a NO donor, showed little effect on BL-induced K+ influx and stomatal opening response in the absence of extracellular Ca2+, but K+ influx and stomatal opening were inhibited by SNP when Ca2+ was added to the bath solution. Interestingly, although both SNP and BL could activate the plasma membrane Ca2+ channels and induce the rise of cytosolic Ca2+, the change in levels of Ca2+ channel activity and cytosolic Ca2+ concentration were different between SNP and BL treatments. SNP at 100 μM obviously activated the plasma membrane Ca2+ channels and induced cytosolic Ca2+ rise by 102.4%. In contrast, a BL pulse (100 μmol/m2 per s for 30 s) slightly activated the Ca2+ channels and resulted in a Ca2+ rise of only 20.8%. Consistently, cytosolic Ca2+ promoted K+ influx at 0.5 μM or below, and significantly inhibited K+ influx at 5 μM or above. Taken together, our findings indicate that Ca2+ plays dual and distinctive roles in the crosstalk between BL and NO signaling in guard cells, mediating both the BL-induced K+ influx as an activator at a lower concentration and the NO-blocked K+ influx as an inhibitor at a higher concentration.

Zhao X, Li YY, Xiao HL, Xu CS, Zhang X (2013) Nitric oxide blocks blue light-induced K+ influx by elevating the cytosolic Ca2+ concentration in Vicia faba L. guard cells. J. Integr. Plant Biol. 55(6), 527–536.

Key words: Blue light, cytosolic Ca2+, nitric oxide, stomatal movement

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