J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (12): 1086-1094.DOI: 10.1111/j.1744-7909.2009.00885.x

Special Issue: Cell Biology and Functions

• Plant-environmental Interactions • Previous Articles     Next Articles

Hydrogen Sulfide Promotes Root Organogenesis in Ipomoea batatas, Salix matsudana and Glycine max

Hua Zhang1*, Jun Tang 2†, Xiao-Ping Liu3, Yun Wang1, Wei Yu1, Wei-Yan Peng1, Fang Fang1, Dai-Fu Ma2*, Zhao-Jun Wei1 and Lan-Ying Hu1   

  1. 1 School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China
    2 Xuzhou Sweet Potato Research Center, Chinese Academy of Agricultural Sciences, National Sweet Potato Improvement Center, Xuzhou 221121, China
    3 Anhui Academy of Agricultural Sciences, Hefei 230031, China †These authors contributed equally to this work.
  • Received:2008-11-09 Accepted:2009-09-13 Published:2009-11-26
  • About author:*Authors for correspondence Tel: +86 551 2901 506 ext. 8635; Fax: +86 551 2901 507; E-mail: zhandzhy@yahoo.com.cn

Abstract:

In this report, we demonstrate that sodium hydrosulfide (NaHS), a hydrogen sulfide (H2S) donor, promoted adventitious root formation mediated by auxin and nitric oxide (NO). Application of the H2S donor to seedling cuttings of sweet potato (Ipomoea batatas L.) promoted the number and length of adventitious roots in a dose-dependent manner. It was also verified that H2S or HS rather than other sulfur-containing components derived from NaHS could be attributed to the stimulation of adventitious root formation. A rapid increase in endogenous H2S, indole acetic acid (IAA) and NO were sequentially observed in shoot tips of sweet potato seedlings treated with HaHS. Further investigation showed that H2S-mediated root formation was alleviated by N-1-naphthylphthalamic acid (NPA), an IAA transport inhibitor, and 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO), an NO scavenger. Similar phenomena in H2S donor-dependent root
organogenesis were observed in both excised willow (Salix matsudana var. tortuosa Vilm) shoots and soybean (Glycine max L.) seedlings. These results indicated that the process of H2S-induced adventitious root formation was likely mediated by IAA and NO, and that H2S acts upstream of IAA and NO signal transduction pathways.
 

Zhang H, Tang J, Liu XP, Wang Y, Yu W, Peng WY, Fang F, Ma DF, Wei ZJ, Hu LY (2009). Hydrogen sulfide promotes root organogenesis in Ipomoea batatas, Salix matsudana and Glycine max. J. Integr. Plant Biol. 51(12), 1086–1094.

Key words: auxin, hydrogen sulfide, nitric oxide, root organogenesis, sweetpotato (Ipomoea batatas).

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