J Integr Plant Biol. ›› 2013, Vol. 55 ›› Issue (8): 735-744.DOI: 10.1111/jipb.12051

• Molecular Physiology • Previous Articles     Next Articles

Genome-wide Association Analysis of Ten Chilling Tolerance Indices at the Germination and Seedling Stages in Maize

Juan Huang, Jianhua Zhang, Wenzhen Li, Wei Hu, Lichao Duan, Yang Feng, Fazhan Qiu and Bing Yue*   

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
  • Received:2012-12-10 Accepted:2013-03-01 Published:2013-08-11
  • About author:*Corresponding author: Tel: +86 27 8728 2689; E-mail: yuebing@webmail.hzau.edu.cn
    Co-Editor: Qian Qian


Maize seedlings are very sensitive to chilling, especially during the transition phase from heterotrophic to autotrophic growth. Genetic dissection of the genetic basis of chilling tolerance would provide useful information for genetic improvement of maize inbreds. In this study, genome-wide association analysis was conducted to explore the genetic architecture of maize chilling tolerance at the seed germination and seedling stages with an association panel of 125 inbreds. Ten tolerance indices (ratios of the performance of 10 germination rates and seedling growth-related traits under chilling stress and control conditions) were investigated to assess the ability of chilling tolerance of the inbreds, and a total of 43 single nucleotide polymorphisms associated with chilling tolerance were detected, with none of them being related to chilling tolerance at both the germination and seedling stages simultaneously. Correlation analysis also revealed that the genetic basis of chilling tolerance at the seed germination stage is generally different from that at the seedling stage. In addition, a total of 40 candidate genes involving 31 of the 43 single nucleotide polymorphisms were predicted, and were grouped into five categories according to their functions. The possible roles of these candidate genes in chilling tolerance were also discussed.

Huang J, Zhang J, Li W, Hu W, Duan L, Feng Y, Qiu F, Yue B (2013) Genome‐wide association analysis of 10 chilling tolerance indices at the germination and seedling stages in maize. J. Integr. Plant Biol. 55(8), 735–744.

Key words: Association mapping, chilling tolerance, germination and seedling stage, maize

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q