J Integr Plant Biol. ›› 2013, Vol. 55 ›› Issue (11): 1038-1053.DOI: 10.1111/jipb.12083

• Metabolism and Biochemistry • Previous Articles     Next Articles

Integration of Metabolomics and Subcellular Organelle Expression Microarray to Increase Understanding the Organic Acid Changes in Post-harvest Citrus Fruit

Xiaohua Sun, Andan Zhu, Shuzhen Liu, Ling Sheng, Qiaoli Ma, Li Zhang, Elsayed Mohamed Elsayed Nishawy, Yunliu Zeng, Juan Xu, Zhaocheng Ma, Yunjiang Cheng* and Xiuxin Deng   

  1. Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, China
  • Received:2013-01-30 Accepted:2013-05-21 Published:2013-11-05
  • About author:*Corresponding author Tel: +86 27 8728 1796; Fax: +86 27 8728 0016; E-mail: yjcheng@mail.hzau.edu.cn

Abstract:

Citric acid plays an important role in fresh fruit flavor and its adaptability to post-harvest storage conditions. In order to explore organic acid regulatory mechanisms in post-harvest citrus fruit, systematic biological analyses were conducted on stored Hirado Buntan Pummelo (HBP; Citrus grandis) fruits. High-performance capillary electrophoresis, subcellular organelle expression microarray, real-time quantitative reverse transcription polymerase chain reaction, gas chromatography mass spectrometry (GC-MS), and conventional physiological and biochemical analyses were undertaken. The results showed that the concentration of organic acids in HBP underwent a regular fluctuation. GC-MS-based metabolic profiling indicated that succinic acid, γ-aminobutyric acid (GABA), and glutamine contents increased, but 2-oxoglutaric acid content declined, which further confirmed that the GABA shunt may have some regulatory roles in organic acid catabolism processes. In addition, the concentration of organic acids was significantly correlated with senescence-related physiological processes, such as hydrogen peroxide content as well as superoxide dismutase and peroxidase activities, which showed that organic acids could be regarded as important parameters for measuring citrus fruit post-harvest senescence processes.

Sun X, Zhu A, Liu S, Sheng L, Ma Q, Zhang L, Nishawy EMS, Zeng Y, Xu J, Ma Z, Cheng Y, Deng X (2013) Integration of metabolomics and subcellular organelle expression microarray increases understanding of the organic acid changes in post‐harvest citrus fruit. J. Integr. Plant Biol. 55(11), 1038–1053.

Key words: Citrus, gene expression, metabolite, organic acids, post-harvest storage

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