J Integr Plant Biol. ›› 2013, Vol. 55 ›› Issue (11): 1026-1037.DOI: 10.1111/jipb.12103

• Cell and Developmental Biology • Previous Articles     Next Articles

Novel Natural Allelic Variations at the Rht-1 Loci in Wheat

Aixia Li1†, Wenlong Yang1†, Xueyuan Lou1, Dongcheng Liu1, Jiazhu Sun1, Xiaoli Guo2, Jing Wang1, Yiwen Li1, Kehui Zhan3, Hong-Qing Ling1 and Aimin Zhang1,3*   

  1. 1The State Key Laboratory of Plant Cell and Chromosome Engineering, National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing, China
    2College of Biological Sciences, China Agricultural University, Beijing, China
    3The Collaborative Innovation Center for Grain Crops in Henan, Henan Agricultural University, Zhengzhou, China
  • Received:2013-08-16 Accepted:2013-08-26 Published:2013-11-05
  • About author:These authors contributed equally to this work.
    *Corresponding author Tel/Fax: +86 10 6480 6618; E-mail: amzhang@genetics.ac.cn

Abstract:

Plant height is an important agronomic trait. Dramatic increase in wheat yield during the “green revolution” is mainly due to the widespread utilization of the Reduced height (Rht)-1 gene. We analyzed the natural allelic variations of three homoeologous loci Rht-A1, Rht-B1, and Rht-D1 in Chinese wheat (Triticum aestivum L.) micro-core collections and the Rht-B1/D1 genotypes in over 1,500 bred cultivars and germplasms using a modified EcoTILLING. We identified six new Rht-A1 allelic variations (Rht-A1b–g), eight new Rht-B1 allelic variations (Rht-B1h–o), and six new Rht-D1 allelic variations (Rht-D1e–j). These allelic variations contain single nucleotide polymorphisms (SNPs) or small insertions and deletions in the coding or uncoding regions, involving two frame-shift mutations and 15 missenses. Of which, Rht-D1e and Rht-D1h resulted in the loss of interactions of GID1-DELLA-GID2, Rht-B1i could increase plant height. We found that the Rht-B1h contains the same SNPs and 197 bp fragment insertion as reported in Rht-B1c. Further detection of Rht-B1h in Tibet wheat germplasms and wheat relatives indicated that Rht-B1c may originate from Rht-B1h. These results suggest rich genetic diversity at the Rht-1 loci and provide new resources for wheat breeding.

Li A, Yang W, Lou X, Liu D, Sun J, Guo X, Wang J, Li Y, Zhan K, Ling HQ, Zhang A (2013) Novel natural allelic variations at the Rht‐1 loci in wheat. J. Integr. Plant Biol. 55(11), 1026–1037.

Key words: Allelic variation, EcoTILLING, Rht-1, wheat, Yeast three hybrid

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