J Integr Plant Biol. ›› 2014, Vol. 56 ›› Issue (3): 281-298.DOI: 10.1111/jipb.12156

• Research Articles • Previous Articles     Next Articles

Nitrate regulates rhizobial and mycorrhizal symbiosis in common bean (Phaseolus vulgaris L.)

Kalpana Nanjareddy1,2, Lourdes Blanco1,2, Manoj-Kumar Arthikala3, Xochitl Alvarado Affantrange3, Federico Sónchez3 and Miguel Lara1,2*   

  1. 1Genómica Funcional de Eucariotes, Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, UNAM, Cuernavaca, Morelos, México
    2Escuela Nacional de Estudios Superiores Unidad León- UNAM, León, Gto. C.P., Mexico
    3Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, UNAM, Cuernavaca, Morelos, México
  • Received:2013-08-29 Accepted:2013-12-31 Published:2014-03-01
  • About author:*Correspondence:
    E-mail: mlara@enes.unam.mx

Abstract:

Nitrogen-limited conditions are considered to be a prerequisite for legume-rhizobial symbiosis, but the effects of nitrate-rich conditions on symbiotic status remain poorly understood. We addressed this issue by examining rhizobial (Rhizobim tropici) and arbusclar mycorrhizal (Glomus intraradices) symbiosis in Phaseolus vulgaris L. cv. Negro Jamapa under nitrate pre-incubation and continuous nitrate conditions. Our results indicate that nitrate pre-incubation, independent of the concentration, did not affect nodule development. However, the continuous supply of nitrate at high concentrations impaired nodule maturation and nodule numbers. Low nitrate conditions, in addition to positively regulating nodule number, biomass, and nitrogenase activity, also extended the span of nitrogen-fixing activity. By contrast, for arbuscular mycorrhizae, continuous 10 and 50 mmol/L nitrate increased the percent root length colonization, concomitantly reduced arbuscule size, and enhanced ammonia transport without affecting phosphate transport. Therefore, in this manuscript, we have proposed the importance of nitrate as a positive regulator in promoting both rhizobial and mycorrhizal symbiosis in the common bean.

Nanjareddy K, Blanco L, Arthikala M‐K, Affantrange XA, Sánchez F, Lara M (2014) Nitrate regulates rhizobial and mycorrhizal symbiosis in common bean (Phaseolus vulgaris L.). J Integr Plant Biol 56: 281–298. doi: 10.1111/jipb.12156

Key words: Arbuscule, common bean, mycorrhiza, nitrate, nodule, Rhizobium

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