J Integr Plant Biol. ›› 2015, Vol. 57 ›› Issue (3): 271-283.DOI: 10.1111/jipb.12286

Special Issue: Abiotic Stresses

• Functional Omics and Systems Biology • Previous Articles     Next Articles

ZmGns, a maize class I β-1,3-glucanase, is induced by biotic stresses and possesses strong antimicrobial activity

Yu-Rong Xie1†, Yenjit Raruang1, Zhi-Yuan Chen1*, Robert L. Brown2 and Thomas E. Cleveland2   

  1. 1Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge, Louisiana, USA
    2Southern Regional Research Center, USDA-ARS, New Orleans, Louisiana, USA
  • Received:2014-08-31 Accepted:2014-09-20 Published:2014-09-23
  • About author:Current address: Biotechnology Research Institute, the Chinese Academy of Agricultural Sciences, Beijing 100081, China
    *Correspondence: E-mail: zchen@agcenter.lsu.edu

Abstract:

Plant β-1,3-glucanases are members of the pathogenesis-related protein 2 (PR-2) family, which is one of the 17 PR protein families and plays important roles in biotic and abiotic stress responses. One of the differentially expressed proteins (spot 842) identified in a recent proteomic comparison between five pairs of closely related maize (Zea mays L.) lines differing in aflatoxin resistance was further investigated in the present study. Here, the corresponding cDNA was cloned from maize and designated as ZmGns. ZmGns encodes a protein of 338 amino acids containing a potential signal peptide. The expression of ZmGns was detectible in all tissues studied with the highest level in silks. ZmGns was significantly induced by biotic stresses including three bacteria and the fungus Aspergillus flavus. ZmGns was also induced by most abiotic stresses tested and growth hormones including salicylic acid. In vivo, ZmGns showed a significant inhibitory activity against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 and fungal pathogen Botrytis cinerea when it overexpressed in Arabidopsis. Its high level of expression in the silk tissue and its induced expression by phytohormone treatment, as well as by bacterial and fungal infections, suggest it plays a complex role in maize growth, development, and defense.

 

Xie YR, Raruang Y, Chen ZY, Brown RL, Cleveland TE (2015). ZmGns, a maize class Ib‐1,3‐glucanase, is induced by biotic stresses and possesses strong antimicrobial activity. J Integr Plant Biol 57: 271–283. doi: 10.1111/jipb.12286

Key words: β, -1,3-glucanase, biological activity, expression profiles, Zea mays, ZmGns

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