J Integr Plant Biol. ›› 2015, Vol. 57 ›› Issue (10): 848-858.DOI: 10.1111/jipb.12329

• Molecular Physiology • Previous Articles     Next Articles

An eukaryotic translation initiation factor, AteIF5A-2, affects cadmium accumulation and sensitivity in Arabidopsis

Xiao-Yan Xu1, Zhong-Jie Ding1, Lei Chen1, Jin-Ying Yan1, Gui-Xin Li2 and Shao-Jian Zheng1*   

  1. 1State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China
    2College of Agronomy and Biotechnology, Zhejiang University, Hangzhou, China
  • Received:2014-11-13 Accepted:2014-12-31 Published:2015-01-05
  • About author:*Correspondence: E-mail: sjzheng@zju.edu.cn

Abstract:

Cadmium (Cd) is one of the most toxic elements and can be accumulated in plants easily; meanwhile, eIF5A is a highly conserved protein in all eukaryotic organisms. The present work tried to investigate whether eIF5A is involved in Cd accumulation and sensitivity in Arabidopsis (Arabidopsis thaliana L.) by comparing the wild-type Columbia-0 (Col-0) with a knockdown mutant of AteIF5A-2, fbr12-3 under Cd stress conditions. The results showed that the mutant fbr12-3 accumulated more Cd in roots and shoots and had significantly lower chlorophyll content, shorter root length, and smaller biomass, suggesting that downregulation of AteIF5A-2 makes the mutant more Cd sensitive. Real-time polymerase chain reaction revealed that the expressions of metal transporters involved in Cd uptake and translocation including IRT1, ZIP1, AtNramp3, and AtHMA4 were significantly increased but the expressions of PCS1 and PCS2 related to Cd detoxification were decreased notably in fbr12-3 compared with Col-0. As a result, an increase in MDA and H2O2 content but decrease in root trolox, glutathione and proline content under Cd stress was observed, indicating that a severer oxidative stress occurs in the mutant. All these results demonstrated for the first time that AteIF5A influences Cd sensitivity by affecting Cd uptake, accumulation, and detoxification in Arabidopsis.

 

Xu XY, Ding ZJ, Chen L, Yan JY, Li GX, Zheng SJ (2015) An eukaryotic translation initiation factor, AteIF5A-2, affects cadmium accumulation and sensitivity in Arabidopsis. J Integr Plant Biol 57: 848–858 doi: 10.1111/jipb.12329

Key words: Arabidopsis, AteIF5A, cadmium toxicity, metal transporter, oxidative stress

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