J Integr Plant Biol. ›› 2015, Vol. 57 ›› Issue (12): 1089-1098.DOI: 10.1111/jipb.12332

Special Issue: Plant Signal Transduction

• Plant Reproduction Biology • Previous Articles    

ABCB1 and ABCB19 auxin transporters have synergistic effects on early and late Arabidopsis anther development

Valentina Cecchetti1,2†, Patrizia Brunetti1,2†, Nadia Napoli1,2, Laura Fattorini3, MariaMaddalena Altamura3, Paolo Costantino2 and Maura Cardarelli1*   

  1. 1IBPM-CNR Institute of Molecular Biology and Pathology, National Research Council, Sapienza University of Rome, Rome, Italy
    2Department of Biology and Biotechnology, Sapienza University of Rome, Rome, Italy
    3Department of Environmental Biology, Sapienza University of Rome, Rome, Italy
  • Received:2014-10-07 Accepted:2015-01-19 Published:2015-01-27
  • About author:These authors contributed equally to this work.
    *Correspondence: E-mail: maura.cardarelli@uniroma1.it

Abstract:

Arabidopsis abcb1 abcb19 double mutants defective in the auxin transporters ABCB1/PGP1 and ABCB19/PGP19 are altered in stamen elongation, anther dehiscence and pollen maturation. To assess the contribution of these transporters to stamen development we performed phenotypic, histological analyses, and in situ hybridizations on abcb1 and abcb19 single mutant flowers. We found that pollen maturation and anther dehiscence are precocious in the abcb1 but not in the abcb19 mutant. Accordingly, endothecium lignification is altered only in abcb1 anthers. Both abcb1 and abcb1 abcb19 stamens also show altered early development, with asynchronous anther locules and a multilayer tapetum. DAPI staining showed that the timing of meiosis is asynchronous in abcb1 abcb19 anther locules, while only a small percentage of pollen grains are non-viable according to Alexander's staining. In agreement, TAM (TARDY ASYNCHRONOUS MEIOSIS), as well as BAM2 (BARELY ANY MERISTEM)—involved in tapetal cell development—are overexpressed in abcb1 abcb19 young flower buds. Correspondingly, ABCB1 and ABCB19 mRNA localization supports the observed phenotypes of abcb1 and abcb1 abcb19 mutant anthers. In conclusion, we provide evidence that auxin transport plays a significant role both in early and late stamen development: ABCB1 plays a major role during anther development, while ABCB19 has a synergistic role.

 

Cecchetti V, Brunetti P, Napoli N, Fattorini L, Altamura MM, Costantino P, Cardarelli M (2015) ABCB1 and ABCB19 auxin transporters have synergistic effects on early and late Arabidopsis anther development. J Integr Plant Biol 57: 1089–1098 doi: 10.1111/jipb.12332

Key words: Anther development, Arabidopsis, auxin transport, endothecium lignification, tapetum differentiation

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