J Integr Plant Biol. ›› 2016, Vol. 58 ›› Issue (1): 67-80.DOI: 10.1111/jipb.12364

Special Issue: Abiotic Stresses

• Molecular Physiology • Previous Articles     Next Articles

Arabidopsis C3HC4-RING finger E3 ubiquitin ligase AtAIRP4 positively regulates stress-responsive abscisic acid signaling

Liang Yang1,2, Qiaohong Liu1, Zhibin Liu1, Hao Yang1, Jianmei Wang1, Xufeng Li1 and Yi Yang1*   

  1. 1Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China
    2The Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China
  • Received:2015-01-31 Accepted:2015-04-23 Published:2015-04-24
  • About author:*Correspondence: E-mail: yangyi528@scu.edu.cn

Abstract:

Degradation of proteins via the ubiquitin system is an important step in many stress signaling pathways in plants. E3 ligases recognize ligand proteins and dictate the high specificity of protein degradation, and thus, play a pivotal role in ubiquitination. Here, we identified a gene, named Arabidopsis thaliana abscisic acid (ABA)-insensitive RING protein 4 (AtAIRP4), which is induced by ABA and other stress treatments. AtAIRP4 encodes a cellular protein with a C3HC4-RING finger domain in its C-terminal side, which has in vitro E3 ligase activity. Loss of AtAIRP4 leads to a decrease in sensitivity of root elongation and stomatal closure to ABA, whereas overexpression of this gene in the T-DNA insertion mutant atairp4 effectively recovered the ABA-associated phenotypes. AtAIRP4 overexpression plants were hypersensitive to salt and osmotic stresses during seed germination, and showed drought avoidance compared with the wild-type and atairp4 mutant plants. In addition, the expression levels of ABA- and drought-induced marker genes in AtAIRP4 overexpression plants were markedly higher than those in the wild-type and atairp4 mutant plants. Hence, these results indicate that AtAIRP4 may act as a positive regulator of ABA-mediated drought avoidance and a negative regulator of salt tolerance in Arabidopsis.

 

Yang L, Liu Q, Liu Z, Yang H, Wang J, Li X, Yang Y (2016) Arabidopsis C3HC4-RING finger E3 ubiquitin ligase AtAIRP4 positively regulates stress-responsive abscisic acid signaling. J Integr Plant Biol 58: 67–80 doi: 10.1111/jipb.12364

Key words: AtAIRP4, abscisic acid, drought stress, E3 ligase, salt stress

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