J Integr Plant Biol. ›› 2016, Vol. 58 ›› Issue (11): 927-940.DOI: 10.1111/jipb.12484

Special Issue: Sexual Reproductions

• Plant Reproduction Biology • Previous Articles    

MARIS plays important roles in Arabidopsis pollen tube and root hair growth

Hong-Ze Liao1, Meng-Meng Zhu2,†, Hong-Hui Cui1, Xin-Yu Du1, Yu Tang1, Li-Qun Chen1, De Ye1 and Xue-Qin Zhang1,*   

  1. 1State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, China
    2Department of Biological Science and Bioengineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, China
  • Received:2016-03-09 Accepted:2016-05-19 Published:2016-05-23
  • About author:Current address: Biology Department, Pennsylvania State University, University Park, Pennsylvania 16802, USA.
    *Correspondence: E-mail: Xue-Qin Zhang (kouyang@cau.edu.cn)

Abstract:

In flowering plants, male gametes are delivered to female gametes for double fertilization through pollen tubes. Therefore, pollen tube growth is crucial for double fertilization. Despite its importance to sexual reproduction, genetic mechanisms of pollen tube growth remain poorly understood. In this study, we characterized the receptor-like cytoplasmic protein kinase (RLCK) gene, MARIS (MRI) that plays critical roles in pollen tube growth. MRI is preferentially expressed in pollen grains, pollen tubes and roots. Mutation in MRI by a Ds insertion led to a burst of pollen tubes after pollen germination. Pollen-rescue assay by pollen and pollen tube-specific expression of MRI in the mri-4 mutant showed that loss of MRI function also severely affected root hair elongation. MRI protein interacted with the protein kinase OXIDATIVE SIGNAL INDUCIBLE1 (OXI1) in the in vitro and in vivo assays, which functions in plant defence and root hair development, and was phosphorylated by OXI1 in vitro. Our results suggest that MRI plays important roles in pollen tube growth and may function in root hair elongation through interaction with OXI1.

Key words: Arabidopsis, pollen tube, protein kinase, receptor-like cytoplasmic protein kinases, root hair

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