J Integr Plant Biol. ›› 2019, Vol. 61 ›› Issue (4): 383-387.DOI: 10.1111/jipb.12726

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REF6 promotes lateral root formation through de-repression of PIN1/3/7 genes

Xiaolei Wang1†, Jiong Gao1†, Shan Gao1,2, Zhongpeng Li1, Benke Kuai1* and Guodong Ren1*   

  1. 1State Key Laboratory of Genetic Engineering and Fudan Center for Genetic Diversity and Designing Agriculture, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200438, China
    2School of Life Sciences, Qilu Normal University, Jinan 250200, China

    These authors contributed equally to this work.
    Email: Guodong Ren (gdren@fudan.edu.cn, Dr. Ren is fully responsible for the distribution of all materials associated with this article); Benke Kuai (bkkuai@fudan.edu.cn)
  • Received:2018-07-01 Accepted:2018-08-31 Online:2018-09-29 Published:2019-04-01

Abstract: The H3K27 methyltransferase CLF inhibits lateral root (LR) formation through depositing the repressive H3K27me3 mark to the chromatin of PIN1, a key polar auxin transporter gene. Here, we show that the H3K27me3 demethylase REF6 promotes lateral root primordium initiation and LR emergence. REF6 directly binds to the chromatin of PIN1/3/7. Dysfunction in REF6 results in increased levels of H3K27me3 on PIN1/3/7 and suppressed expression of PIN genes. Genetic analysis of the clf ref6 double mutant revealed an antagonistic action between CLF and REF6, in terms of LR formation. Our findings indicate that H3K27 methylation and demethylation activities are likely coordinated to ensure proper LR organogenesis.

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