J Integr Plant Biol ›› 2019, Vol. 61 ›› Issue (9): 1000-1014.DOI: 10.1111/jipb.12739

• Cell and Developmental Biology • Previous Articles     Next Articles

A leaf shape mutant provides insight into PINOID Serine/Threonine Kinase function in cucumber (Cucumis sativus L.)

Mengfei Song, Feng Cheng, Jing Wang, Qingzhen Wei, Wenyuan Fu, Xiaqing Yu, Ji Li, Jinfeng Chen* and Qunfeng Lou*   

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China

    These authors contributed equally to the work.
    *Correspondences:
    Email: Jinfeng Chen (jfchen@njau.edu.cn); Qunfeng Lou (qflou@njau.edu.cn). Dr. Lou is fully responsible for the distribution of all materials associated with this article.
  • Received:2018-09-21 Accepted:2018-11-08 Online:2018-11-12 Published:2019-09-01

Abstract:

Optimizing leaf shape is a major challenge in efforts to develop an ideal plant type. Cucumber leaf shapes are diverse; however, the molecular regulatory mechanisms underlying leaf shape formation are unknown. In this study, we obtained a round leaf mutant (rl) from an ethyl methanesulfonate‐induced mutagenesis population. Genetic analysis revealed that a single recessive gene, rl, is responsible for this mutation. A modified MutMap analysis combined linkage mapping identified a single nucleotide polymorphism within a candidate gene, Csa1M537400, as the mutation underlying the trait. Csa1M537400 encodes a PINOID kinase protein involved in auxin transport. Expression of Csa1M537400 was significantly lower in the rl mutant than in wild type, and it displayed higher levels of IAA (indole‐3‐acetic acid) in several tissues. Treatment of wild‐type plants with an auxin transport inhibitor induced the formation of round leaves, similar to those in the rl mutant. Altered expression patterns of several auxin‐related genes in the rl mutant suggest that rl plays a key role in auxin biosynthesis, transport, and response in cucumber. These findings provide insight into the molecular mechanism underlying the regulation of auxin signaling pathways in cucumber, and will be valuable in the development of an ideal plant type.

We obtained a round leaf mutant (rl) from an ethyl methanesulfonate (EMS)‐induced mutagenesis population in cucumber. Genetic analysis revealed that a single recessive gene, Csa1M537400, is responsible for this mutation. Csa1M537400 encodes a PINOID kinase protein, and plays a key role in auxin biosynthesis, transport, and response in cucumber.
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