J Integr Plant Biol. ›› 2020, Vol. 62 ›› Issue (4): 403-420.DOI: 10.1111/jipb.12817

• Cell and Developmental Biology • Previous Articles     Next Articles

The Brassicaceae-specific secreted peptides, STMPs, function in plant growth and pathogen defense

Zipeng Yu1†, Yang Xu1,2†, Lifei Zhu1, Lei Zhang1, Lin Liu1, Di Zhang1, Dandan Li1, Changai Wu1, Jinguang Huang1, Guodong Yang1, Kang Yan1, Shizhong Zhang1* and Chengchao Zheng1*   

  1. 1State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, China
    2Shandong Peanut Research Institute, Shandong Academy of Agricultural Sciences, Qingdao 266100, China

    These authors contributed equally to this work.
    *Correspondences:
    Email: Chengchao Zheng (cczheng@sdau.edu.cn, Dr. Zheng is fully responsible for the distribution of all materials associated with this article); Shizhong Zhang (shizhong@sdau.edu.cn)
  • Received:2019-01-17 Accepted:2019-04-11 Online:2019-04-19 Published:2020-04-01

Abstract:

Low molecular weight secreted peptides have recently been shown to affect multiple aspects of plant growth, development, and defense responses. Here, we performed stepwise BLAST filtering to identify unannotated peptides from the Arabidopsis thaliana protein database and uncovered a novel secreted peptide family, secreted transmembrane peptides (STMPs). These low molecular weight peptides, which consist of an N‐terminal signal peptide and a transmembrane domain, were primarily localized to extracellular compartments but were also detected in the endomembrane system of the secretory pathway, including the endoplasmic reticulum and Golgi. Comprehensive bioinformatics analysis identified 10 STMP family members that are specific to the Brassicaceae family. Brassicaceae plants showed dramatically inhibited root growth upon exposure to chemically synthesized STMP1 and STMP2. Arabidopsis overexpressing STMP1, 2, 4, 6, or 10 exhibited severely arrested growth, suggesting that STMPs are involved in regulating plant growth and development. In addition, in vitro bioassays demonstrated that STMP1, STMP2, and STMP10 have antibacterial effects against Pseudomonas syringae pv. tomato DC3000, Ralstonia solanacearum, Bacillus subtilis, and Agrobacterium tumefaciens, demonstrating that STMPs are antimicrobial peptides. These findings suggest that STMP family members play important roles in various developmental events and pathogen defense responses in Brassicaceae plants.

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