J Integr Plant Biol. ›› 2020, Vol. 62 ›› Issue (10): 1594-1606.DOI: 10.1111/jipb.12924

• Plant Reproduction Biology • Previous Articles     Next Articles

A novel CCCH‐type zinc finger protein SAW1 activates OsGA20ox3 to regulate gibberellin homeostasis and anther development in rice

Bin Wang1, 2, 3, 4†, Ruiqiu Fang1, 2, 5†, Faming Chen1, 4, Jingluan Han1, 2, 3, 4, Yao-Guang Liu1, 2, 4, Letian Chen1, 2, 3, 4* and Qinlong Zhu1, 2, 4*   

  1. 1State Key Laboratory for Conservation and Utilization of Subtropical Agro‐Bioresources, South China Agricultural University, Guangzhou 510642, China
    2Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China
    3Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, South China Agricultural University, Guangzhou 510642, China
    4College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
    5Dongyang Institute of Maize Research, Zhejiang Academy of Agricultural Sciences, Dongyang 322100, China

    These authors contributed equally to this work.
    *Correspondences:
    Email: Letian Chen (lotichen@scau.edu.cn, Dr. Chen is fully responsible for the distribution of materials associated with this article); Qinlong Zhu (zhuql@scau.edu.cn)
  • Received:2020-01-05 Accepted:2020-03-06 Online:2020-03-09 Published:2020-10-01

Abstract:

Male sterility is a prerequisite for hybrid seed production. The phytohormone gibberellin (GA) is involved in regulating male reproductive development, but the mechanism underlying GA homeostasis in anther development remains less understood. Here, we report the isolation and characterization of a new positive regulator of GA homeostasis, swollen anther wall 1 (SAW1), for anther development in rice (Oryza sativa L.). Rice plants carrying the recessive mutant allele saw1 produces abnormal anthers with swollen anther wall and aborted pollen. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRIPSR‐associated protein 9‐mediated knockout of SAW1 in rice generated similar male sterile plants. SAW1 encodes a novel nucleus‐localizing CCCH‐tandem zinc finger protein, and this protein could directly bind to the promoter region of the GA synthesis gene OsGA20ox3 to induce its anther‐specific expression. In the saw1 anther, the significantly decreased OsGA20ox3 expression resulted in lower bioactive GA content, which in turn caused the lower expression of the GA‐inducible anther‐regulator gene OsGAMYB. Thus, our results disclose the mechanism of the SAW1GA20ox3‐GAMYB pathway in controlling rice anther development, and provide a new target gene for the rapid generation of male sterile lines by genome editing for hybrid breeding.

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