J Integr Plant Biol. ›› 2021, Vol. 63 ›› Issue (2): 378-392.DOI: 10.1111/jipb.13025

• Research Articles • Previous Articles     Next Articles

ANNEXIN 8 negatively regulates RPW8.1‐mediated cell death and disease resistance in Arabidopsis

Zhi‐Xue Zhao1†, Yong‐Ju Xu1†, Yang Lei1†, Qin Li1†, Ji‐Qun Zhao1, Yan Li1, Jing Fan1, Shunyuan Xiao2 and Wen‐Ming Wang1*   

  1. 1Rice Research Institute and Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, China
    2Institute for Bioscience and Biotechnology Research & Department of Plant Science and Landscape Architecture, University of Maryland, Rockville, Maryland 20850, USA

    These authors contributed equally to this work
    *Corresponding author: Wen-Ming Wang (j316wenmingwang@sicau.edu.cn)
  • Received:2020-06-25 Accepted:2020-10-15 Online:2020-10-19 Published:2021-02-01

Abstract: Study on the regulation of broad‐spectrum resistance is an active area in plant biology. RESISTANCE TO POWDERY MILDEW 8.1 (RPW8.1) is one of a few broad‐spectrum resistance genes triggering the hypersensitive response (HR) to restrict multiple pathogenic infections. To address the question how RPW8.1 signaling is regulated, we performed a genetic screen and tried to identify mutations enhancing RPW8.1‐mediated HR. Here, we provided evidence to connect an annexin protein with RPW8.1‐mediated resistance in Arabidopsis against powdery mildew. We isolated and characterized Arabidopsis b7‐6 mutant. A point mutation in b7‐6 at the At5g12380 locus resulted in an amino acid substitution in ANNEXIN 8 (AtANN8). Loss‐of‐function or RNA‐silencing of AtANN8 led to enhanced expression of RPW8.1, RPW8.1‐dependent necrotic lesions in leaves, and defense against powdery mildew. Conversely, over‐expression of AtANN8 compromised RPW8.1‐mediated disease resistance and cell death. Interestingly, the mutation in AtANN8 enhanced RPW8.1‐triggered H2O2. In addition, mutation in AtANN8 led to hypersensitivity to salt stress. Together, our data indicate that AtANN8 is involved in multiple stress signaling pathways and negatively regulates RPW8.1‐mediated resistance against powdery mildew and cell death, thus linking ANNEXIN's function with plant immunity.

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