J Integr Plant Biol. ›› 2021, Vol. 63 ›› Issue (5): 937-948.DOI: 10.1111/jipb.13048

Special Issue: Development Hormone signaling

• Plant-abiotic Interactions • Previous Articles     Next Articles

Induction of jasmonic acid biosynthetic genes inhibits Arabidopsis growth in response to low boron

Yupu Huang1,2, Sheliang Wang1,2*, Chuang Wang2, Guangda Ding1,2, Hongmei Cai2, Lei Shi1,2 and Fangsen Xu1,2*   

  1. 1National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
    2Microelement Research Center, College of Resources & Environment, Huazhong Agricultural University, Wuhan 430070, China

    *Correspondences: Fangsen Xu (fangsenxu@mail.hzau.edu.cn, Dr. Xu is responsible for the distributions of all materials associated with this article); Sheliang Wang (sheliangwang2017@mail.hzau.edu.cn)
  • Received:2020-09-19 Accepted:2020-12-02 Online:2020-12-07 Published:2021-05-01

Abstract: The essential micronutrient boron (B) has key roles in cell wall integrity and B deficiency inhibits plant growth. The role of jasmonic acid (JA) in plant growth inhibition under B deficiency remains unclear. Here, we report that low B elevates JA biosynthesis in Arabidopsis thaliana by inducing the expression of JA biosynthesis genes. Treatment with JA inhibited plant growth and, a JA biosynthesis inhibitor enhanced plant growth, indicating that the JA induced by B deficiency affects plant growth. Furthermore, examination of the JA signaling mutants jasmonate resistant1, coronatine insensitive1‐2, and myc2 showed that JA signaling negatively regulates plant growth under B deficiency. We identified a low‐B responsive transcription factor, ERF018, and used yeast one‐hybrid assays and transient activation assays in Nicotiana benthamiana leaf cells to demonstrate that ERF018 activates the expression of JA biosynthesis genes. ERF018 overexpression (OE) lines displayed stunted growth and up‐regulation of JA biosynthesis genes under normal B conditions, compared to Col‐0 and the difference between ERF018 OE lines and Col‐0 diminished under low B. These results suggest that ERF018 enhances JA biosynthesis and thus negatively regulates plant growth. Taken together, our results highlight the importance of JA in the effect of low B on plant growth.

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