J Integr Plant Biol. ›› 2021, Vol. 63 ›› Issue (3): 494-509.DOI: 10.1111/jipb.13058

Special Issue: Abiotic stress Hormone signaling Protein kinases

• Research Articles • Previous Articles     Next Articles

Arabidopsis U‐box E3 ubiquitin ligase PUB11 negatively regulates drought tolerance by degrading the receptor‐like protein kinases LRR1 and KIN7

Xuexue Chen1, Tingting Wang1, Amin Ur Rehman1, Yu Wang1, Junsheng Qi1, Zhen Li1, Chunpeng Song2, Baoshan Wang3, Shuhua Yang1 and Zhizhong Gong1,4*   

  1. 1State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China
    2Collaborative Innovation Center of Crop Stress Biology, Institute of Plant Stress Biology, Henan University, Kaifeng 475001, China
    3Key Lab of Plant Stress Research, College of Life Science, Shandong Normal University, Ji'nan 250000, China
    4School of Life Sciences, Institute of Life Science and Green Development, Hebei University, Baoding 100193, China

    *Correspondence: Zhizhong Gong (gongzz@cau.edu.cn)
  • Received:2020-10-16 Accepted:2020-12-17 Online:2020-12-21 Published:2021-03-01

Abstract: Both plant receptor‐like protein kinases (RLKs) and ubiquitin‐mediated proteolysis play crucial roles in plant responses to drought stress. However, the mechanism by which E3 ubiquitin ligases modulate RLKs is poorly understood. In this study, we showed that Arabidopsis PLANT U‐BOX PROTEIN 11 (PUB11), an E3 ubiquitin ligase, negatively regulates abscisic acid (ABA)‐mediated drought responses. PUB11 interacts with and ubiquitinates two receptor‐like protein kinases, LEUCINE RICH REPEAT PROTEIN 1 (LRR1) and KINASE 7 (KIN7), and mediates their degradation during plant responses to drought stress in vitro and in vivo. pub11 mutants were more tolerant, whereas lrr1 and kin7 mutants were more sensitive, to drought stress than the wild type. Genetic analyses show that the pub11 lrr1 kin7 triple mutant exhibited similar drought sensitivity as the lrr1 kin7 double mutant, placing PUB11 upstream of the two RLKs. Abscisic acid and drought treatment promoted the accumulation of PUB11, which likely accelerates LRR1 and KIN7 degradation. Together, our results reveal that PUB11 negatively regulates plant responses to drought stress by destabilizing the LRR1 and KIN7 RLKs.

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