J Integr Plant Biol. ›› 2021, Vol. 63 ›› Issue (8): 1568-1587.DOI: 10.1111/jipb.13100

Special Issue: Crop yield Protein kinases Temperature signaling

• Plant-abiotic Interactions • Previous Articles    

Reciprocal regulation between the negative regulator PP2CG1 phosphatase and the positive regulator OST1 kinase confers cold response in Arabidopsis

Jian Lv1, Jingyan Liu1, Yuhang Ming1, Yiting Shi1, Chunpeng Song2, Zhizhong Gong1, Shuhua Yang1 and Yanglin Ding1*   

  1. 1 State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China
    2 Institute of Plant Stress Biology, Collaborative Innovation Center of Crop Stress Biology, Henan University, Kaifeng 475001, China

    *Correspondence: Yanglin Ding (ding_yanglin@cau.edu.cn)
  • Received:2021-04-07 Accepted:2021-04-18 Online:2021-04-19 Published:2021-08-01

Abstract: Protein phosphorylation and dephosphorylation have been reported to play important roles in plant cold responses. In addition, phospho-regulatory feedback is a conserved mechanism for biological processes and stress responses in animals and plants. However, it is less well known that a regulatory feedback loop is formed by the protein kinase and the protein phosphatase in plant responses to cold stress. Here, we report that OPEN STOMATA 1 (OST1) and PROTEIN PHOSPHATASE 2C G GROUP 1 (PP2CG1) reciprocally regulate the activity during the cold stress response. The interaction of PP2CG1 and OST1 is inhibited by cold stress, which results in the release of OST1 at the cytoplasm and nucleus from suppression by PP2CG1. Interestingly, cold-activated OST1 phosphorylates PP2CG1 to suppress its phosphatase activity, thereby amplifying cold signaling in plants. Mutations of PP2CG1 and its homolog PP2CG2 enhance freezing tolerance, whereas overexpression of PP2CG1 decreases freezing tolerance. Moreover, PP2CG1 negatively regulates protein levels of C-REPEAT BINDING FACTORs (CBFs) under cold stress. Our results uncover a phosphor/dephosphor-regulatory feedback loop mediated by PP2CG1 phosphatase and OST1 protein kinase in plant cold responses.

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