J Integr Plant Biol.

• Research Articles •    

Rice SPL10 positively regulates trichome development through expression of HL6 and auxin-related genes

Jinjie Li1†*, Bo Tang1†, Yingxiu Li1†, Chenguang Li1†, Minjie Guo1, Haiyang Chen1, Shichen Han1, Jin Li1, Qijin Lou1, Wenqiang Sun2, Peng Wang3, Haifeng Guo1, Wei Ye1, Zhanying Zhang1, Hongliang Zhang1, Sibin Yu2, Long Zhang3 and Zichao Li1*   

  1. 1State Key Laboratory of Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China
    2National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
    3College of Plant Protection, China Agricultural University, Beijing 100193, China

    These authors contributed equally to this work.
    *Correspondence: Jinjie Li (lijinjie@cau.edu.cn, Dr. Li is fully responsible for the distribution of all materials associated with this article); Zichao Li (lizichao@cau.edu.cn)
  • Received:2021-03-24 Accepted:2021-05-24 Online:2021-05-26

Abstract: Trichomes function in plant defenses against biotic and abiotic stresses; examination of glabrous lines, which lack trichomes, has revealed key aspects of trichome development and function. Tests of allelism in 51 glabrous rice (Oryza sativa) accessions collected worldwide identified OsSPL10 and OsWOX3B as regulators of trichome development in rice. Here, we report that OsSPL10 acts as a transcriptional regulator controlling trichome development. Haplotype and transient expression analyses revealed that variation in the ~700-bp OsSPL10 promoter region is the primary cause of the glabrous phenotype in the indica cultivar WD-17993. Disruption of OsSPL10 by genome editing decreased leaf trichome density and length in the NIL-HL6 background. Plants with genotype OsSPL10WD-17993/HL6 generated by crossing WD-17993 with NIL-HL6 also had fewer trichomes in the glumes. HAIRY LEAF6 (HL6) encodes another transcription factor that regulates trichome initiation and elongation, and OsSPL10 directly binds to the HL6 promoter to regulate its expression. Moreover, the transcript levels of auxin-related genes, such as OsYUCCA5 and OsPIN-FORMED1b, were altered in OsSPL10 overexpression and RNAi transgenic lines. Feeding tests using locusts (Locusta migratoria) demonstrated that non-glandular trichomes affect feeding by this herbivore. Our findings provide a molecular framework for trichome development and an ecological perspective on trichome functions.

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