J Integr Plant Biol. ›› 2022, Vol. 64 ›› Issue (10): 1916-1934.DOI: 10.1111/jipb.13341

• Cell and Developmental Biology • Previous Articles     Next Articles

The unconventional prefoldin RPB5 interactor mediates the gravitropic response by modulating cytoskeleton organization and auxin transport in Arabidopsis

Yi Yang1,2,3, Fang Liu1,4, Le Liu4, Mingyue Zhu1,3, Jinfeng Yuan1,3, Yan‐Xia Mai5, Jun‐Jie Zou6, Jie Le7,8, Yonghong Wang1, Klaus Palme3,4, Xugang Li1,3*, Yong Wang1* and Long Wang5*   

  1. 1 State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Daizong Street 61, Tai'an 271018, China
    2 Shandong Provincial Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou 253023, China
    3 Sino‐German Joint Research Center on Agricultural Biology, College of Life Sciences, Shandong Agricultural University, Daizong Street 61, Tai'an 271018, China
    4 Faculty of Biology, Institute of Biology II/Molecular Plant Physiology, Albert‐Ludwigs‐University of Freiburg, Schänzlestrasse 1Freiburg D‐79104, Germany
    5 National Key Laboratory of Plant Molecular Genetics (NKLPMG), CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology (SIPPE), Chinese Academy of Sciences (CAS), Shanghai 200032, China
    6 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    7 Key Laboratory of Plant Molecular Physiology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
    8 University of Chinese Academy of Sciences, Beijing 100049, China

    *Correspondences: Long Wang (longwang@cemps.ac.cn, Dr. Wang is fully responsible for the distribution of the materials associated with this article); Yong Wang (wangyong@sdau.edu.cn); Xugang Li (xgli@sdau.edu.cn)
  • Received:2022-05-23 Accepted:2022-08-08 Online:2022-08-09 Published:2022-10-01

Abstract: Gravity-induced root curvature involves the asymmetric distribution of the phytohormone auxin. This response depends on the concerted activities of the auxin transporters such as PIN-FORMED (PIN) proteins for auxin efflux and AUXIN RESISTANT 1 (AUX1) for auxin influx. However, how the auxin gradient is established remains elusive. Here we identified a new mutant with a short root, strong auxin distribution in the lateral root cap and an impaired gravitropic response. The causal gene encoded an Arabidopsis homolog of the human unconventional prefoldin RPB5 interactor (URI). AtURI interacted with prefoldin 2 (PFD2) and PFD6, two β-type PFD members that modulate actin and tubulin patterning in roots. The auxin reporter DR5rev:GFP showed that asymmetric auxin redistribution after gravistimulation is disordered in aturi-1 root tips. Treatment with the endomembrane protein trafficking inhibitor brefeldin A indicated that recycling of the auxin transporter PIN2 is disrupted in aturi-1 roots as well as in pfd mutants. We propose that AtURI cooperates with PFDs to recycle PIN2 and modulate auxin distribution.

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