J Integr Plant Biol. ›› 2022, Vol. 64 ›› Issue (11): 2029-2032.DOI: 10.1111/jipb.13352

• Brief Communications •     Next Articles

Base editing-mediated targeted evolution of ACCase for herbicide-resistant rice mutants

Hongzhi Wang1,2†, Yuxin He1,2†, Yingying Wang1,2†, Zuren Li3, Jiannan Hao1,2, Yijiao Song4, Mugui Wang1* and Jian‐Kang Zhu1,5,6*   

  1. 1 Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China
    2 University of Chinese Academy of Sciences, Beijing 100049, China
    3 Hunan Provincial Key Laboratory for Biology and Control of Weeds, Hunan Academy of Agricultural Sciences, Changsha 410125, China
    4 College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
    5 Institute of Advanced Biotechnology and School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China
    6 Center for Advanced Bioindustry Technologies, Chinese Academy of Agricultural Sciences, Beijing 100081, China

    These authors contributed equally to this work.
    * Correspondences: Jian‐Kang Zhu (zhujk@sustech.edu.cn, Dr. Zhu is fully responsible for the distribution of the materials associated with this article); Mugui Wang (mgwang@cemps. ac.cn)
  • Received:2022-06-16 Accepted:2022-08-26 Online:2022-08-29 Published:2022-11-01

Abstract: Improved cytosine and adenine base editors and an efficient dual editor were applied in targeted evolution of ACETYL COA CARBOXYLASE in rice, resulting in the generation of dozens of herbicide-resistant mutations, at least three of which, W2125L, W2125Q and C2186H, have not been reported previously.

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