J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (1): 265-279.DOI: 10.1111/jipb.13403

• Plant Biotic Interactions • Previous Articles    

Plasma membrane-localized Hsp40/DNAJ chaperone protein facilitates OsSUVH7-OsBAG4-OsMYB106 transcriptional complex formation for OsHKT1;5 activation

Yutong Liu1†, Mengting Li1†, Jinlei Yu1, Ao Ma1, Jie Wang1, Dae-Jin Yun1,2, Zheng-Yi Xu1*   

  1. 1. Key Laboratory of Molecular Epigenetics of the Ministry of EducationMOE) Northeast Normal University Changchun 130024 China;
    2. Department of Biomedical Science and Engineering Konkuk University Seoul South Korea
    These authors contributed equally to this work.
    *Correspondence: Zheng‐Yi Xu (xuzy100@nenu.edu.cn)
  • Received:2022-10-10 Accepted:2022-11-07 Online:2022-11-11 Published:2023-01-01

Abstract: The salinization of irrigated land affects agricultural productivity. HIGH-AFFINITY POTASSIUM (K+) TRANSPORTER 1;5 (OsHKT1;5)-dependent sodium (Na+) transport is a key salt tolerance mechanism during rice growth and development. Using a previously generated high-throughput activation tagging-based T-DNA insertion mutant pool, we isolated a mutant exhibiting salt stress-sensitive phenotype, caused by a reduction in OsHKT1;5 transcripts. The salt stress-sensitive phenotype of this mutant results from the loss of function of OsDNAJ15, which encodes plasma membrane-localized heat shock protein 40 (Hsp40). osdnaj15 loss-of-function mutants show decreased plant height, increased leaf angle, and reduced grain number caused by shorter panicle length and fewer branches. On the other h'and, OsDNAJ15-overexpression plants showed salt stress-tolerant phenotypes. Intriguingly, salt stress facilitates the nuclear relocation of OsDNAJ15 so that it can interact with OsBAG4, and OsDNAJ15 and OsBAG4 synergistically facilitate the DNA-binding activity of OsMYB106 to positively regulate the expression of OsHKT1;5. Overall, our results reveal a novel function of plasma membrane-localized Hsp40 protein in modulating, alongside chaperon regulator OsBAG4, transcriptional regulation under salinity stress tolerance.

Key words: chaperon regulator, Oryza sativa, small heat shock protein, sodium transport, transcriptional regulation

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