J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (7): 1613-1619.DOI: 10.1111/jipb.13472

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CPR5 positively regulates pattern-triggered immunity via a mediator protein

Miaomiao Ma1,2,3†, Meng Li1†, Rongfang Zhou1‡, Jian‐Bin Yu4, Ying Wu1, Xiaojuan Zhang1,2, Jinlong Wang1‡, Jian‐Min Zhou1,2,5* and Xiangxiu Liang4*   

  1. 1. State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100101, China;
    2. CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, China;
    3. College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China;
    4. State Key Laboratory for Conservation and Utilization of Subtropical Agro‐bioresources, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China;
    5. Hainan Yazhou Bay Seed Laboratory, Sanya 572025, China
    These authors contributed equally to this work.
    Addresses: Rongfang Zhou, Wuhan BGI Technology Co., Ltd, 430000, China; Jinlong Wang, Department of Biology, Duke University, Durham, North Carolina 27708, USA
    *Correspondences: Jian‐Min Zhou (jmzhou@genetics.ac.cn); Xiangxiu Liang (liangxiangxiu@scau.edu.cn, Dr. Liang is fully responsible for the distributions of the materials associated with this article)
  • Received:2022-11-27 Accepted:2023-02-26 Online:2023-03-01 Published:2023-07-01

Abstract: Plant cells possess a two-layered immune system consisting of pattern-triggered immunity (PTI) and effector-triggered immunity (ETI), mediated by cell surface pattern-recognition receptors and intracellular nucleotide-binding leucine-rich repeat receptors (NLRs), respectively. The CONSTITUTIVE EXPRESSION OF PR GENES 5 (CPR5) nuclear pore complex protein negatively regulates ETI, including ETI-associated hypersensitive response. Here, we show that CPR5 is essential for the activation of various PTI responses in Arabidopsis, such as resistance to the non-adapted bacterium Pseudomonas syringae pv. tomato DC3000 hrcC-. In a forward-genetic screen for suppressors of cpr5, we identified the mediator protein MED4. Mutation of MED4 in cpr5 greatly restored the defective PTI of cpr5. Our findings reveal that CPR5 plays opposite roles in regulating PTI and ETI, and genetically regulates PTI via MED4.

Key words: CPR5, MED4, plant immunity, PTI

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