Epigenetic regulation of N-hydroxypipecolic acid biosynthesis by the AIPP3-PHD2-CPL2 complex

doi:10.1111/jipb.13577

J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (12): 2660-2671.DOI: 10.1111/jipb.13577

• Plant Biotic Interactions • Previous Articles Next Articles

Epigenetic regulation of N-hydroxypipecolic acid biosynthesis by the AIPP3-PHD2-CPL2 complex

Jiameng Lan¹, Siyu Chen^1,2, Joana Pico³, Kevin Ao^1,4, Shitou Xia⁵, Shucai Wang², Xin Li^1,4, Simone D. Castellarin³ and Yuelin Zhang^1,6*

1. Department of Botany, University of British Columbia, Vancouver V6T 1Z4, Canada;
2. Laboratory of Plant Molecular Genetics&Crop Gene Editing, School of Life Sciences, Linyi University, Linyi 276005, China;
3. Wine Research Centre, Faculty of Land and Food Systems, University of British Columbia, Vancouver V6T 1Z4, Canada;
4. Michael Smith Laboratories, University of British Columbia, Vancouver V6T 1Z4, Canada;
5. Hunan Provincial Key Laboratory of Phytohormones, Hunan Agricultural University, Changsha 410125, China;
6. College of Life Science, Sichuan University, Chengdu 610064, China
^*Correspondence:Yuelin Zhang(yuelin.zhang@scu.edu.cn)

Received:2023-06-04 Accepted:2023-10-23 Online:2023-10-22 Published:2023-12-01

Abstract

Abstract: N-Hydroxypipecolic acid (NHP) is a signaling molecule crucial for systemic acquired resistance (SAR), a systemic immune response in plants that provides long-lasting and broad-spectrum protection against secondary pathogen infections. To identify negative regulators of NHP biosynthesis, we performed a forward genetic screen to search for mutants with elevated expression of the NHP biosynthesis gene FLAVIN-DEPENDENT MONOOXYGENASE 1 (FMO1). Analysis of two constitutive expression of FMO1 (cef) and one induced expression of FMO1 (ief) mutants revealed that the AIPP3-PHD2-CPL2 protein complex, which is involved in the recognition of the histone modification H3K27me3 and transcriptional repression, contributes to the negative regulation of FMO1 expression and NHP biosynthesis. Our study suggests that epigenetic regulation plays a crucial role in controlling FMO1 expression and NHP levels in plants.

Key words: plant immunity, SAR, epigenetic regulation, NHP, FMO1, H3K27me3, transcriptional repression, AIPP3, PHD2, CPL2

Jiameng Lan, Siyu Chen, Joana Pico, Kevin Ao, Shitou Xia, Shucai Wang, Xin Li, Simone D. Castellarin and Yuelin Zhang. Epigenetic regulation of N-hydroxypipecolic acid biosynthesis by the AIPP3-PHD2-CPL2 complex[J]. J Integr Plant Biol., 2023, 65(12): 2660-2671.

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Epigenetic regulation of N-hydroxypipecolic acid biosynthesis by the AIPP3-PHD2-CPL2 complex

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