J Integr Plant Biol.

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UVR8‐TCP4‐LOX2 module regulates UV‐B tolerance in Arabidopsis

Cheng Li1,2†, Jiancan Du1,3†, Huini Xu4, Zhenhua Feng1, Caspar C. C. Chater5, Yuanwen Duan1*, Yongping Yang1* and Xudong Sun1*   

  1. 1. Yunnan Key Laboratory of Crop Wild Relatives Omics, The Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China;
    3. CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China;
    4. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650031, China;
    5. Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3AE, UK
    These authors contributed equally to this work.
    *Correspondences: Yuanwen Duan (duanyw@mail.kib.ac.cn); Yongping Yang (yangyp@mail.kib.ac.cn); Xudong Sun (sunxudong@mail.kib.ac.cn, Dr. Sun is fully responsible for the distributions of all materials associated with this article)
  • Received:2023-11-16 Accepted:2024-03-01 Online:2024-03-20

Abstract: The phytohormone jasmonate (JA) coordinates stress and growth responses to increase plant survival in unfavorable environments. Although JA can enhance plant UV-B stress tolerance, the mechanisms underlying the interaction of UV-B and JA in this response remain unknown. In this study, we demonstrate that the UV RESISTANCE LOCUS 8 - TEOSINTE BRANCHED1, Cycloidea and PCF 4 - LIPOXYGENASE2 (UVR8-TCP4-LOX2) module regulates UV-B tolerance dependent on JA signaling pathway in Arabidopsis thaliana. We show that the nucleus-localized UVR8 physically interacts with TCP4 to increase the DNA-binding activity of TCP4 and upregulate the JA biosynthesis gene LOX2. Furthermore, UVR8 activates the expression of LOX2 in a TCP4-dependent manner. Our genetic analysis also provides evidence that TCP4 acts downstream of UVR8 and upstream of LOX2 to mediate plant responses to UV-B stress. Our results illustrate that the UV-B-dependent interaction of UVR8 and TCP4 serves as an important UVR8-TCP4-LOX2 module, which integrates UV-B radiation and JA signaling and represents a new UVR8 signaling mechanism in plants.

Key words: anthocyanin accumulation, jasmonic acid, TCP4, UV‐B, UVR8

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