J Integr Plant Biol.

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Transcriptional regulation of phospholipid transport in cotton fiber elongation by GhMYB30D04–GhHD1 interaction complex

Qingwei Song1, Chuanhui Du1, Yiyang Xu1, Jin Wang2, Min Lin3 and Kaijing Zuo1   

  1. 1. Single Cell Research Center, College of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
    2. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    3. College of Agriculture, Henan University, Kaifeng 450046, China
    *Correspondence: Kaijing Zuo, Single Cell Research Center, College of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China. (kjzuo@sjtu.edu.cn)
  • Received:2024-02-15 Accepted:2024-08-21 Online:2024-09-18
  • Supported by:
    This work was funded by Biological Breeding‐Major Projects (2023ZD04076), National Science Foundation of China (32370267).

Abstract: Cotton fiber length is basically determined by well-coordinated gene expression and phosphatidylinositol phosphates (PIPs) accumulation during fiber elongation but the regulatory mechanism governing PIPs transport remains unknown. Here, we report a MYB transcription factor GhMYB30D04 in Gossypium hirsutum that promotes fiber elongation through modulating the expression of PIP transporter gene GhLTPG1. Knockout of GhMYB30D04 gene in cotton (KO) results in a reduction of GhLTPG1 transcripts with lower accumulation of PIPs, leading to shorter fibers and lower fiber yield. Conversely, GhMYB30D04 overexpression (GhMYB30D04-OE) causes richer PIPs and longer cotton fibers, mimicking the effects of exogenously applying PIPs on the ovules of GhMYB30D04-KO and wild type. Furthermore, GhMYB30D04 interacts with GhHD1, the crucial transcription factor of fiber initiation, to form an activation complex stabilized by PIPs, both of which upregulate GhLTPG1 expression. Comparative omics-analysis revealed that higher and extended expressions of LTPG1 in fiber elongation mainly correlate with the variations of the GhMYB30D04 gene between two cotton allotetraploids, contributing to longer fiber in G. babardense. Our work clarifies a mechanism by which GhHD1–GhMYB30D04 form a regulatory module of fiber elongation to tightly control PIP accumulation. Our work still has an implication that GhMYB30D04–GhHD1 associates with development transition from fiber initiation to elongation.

Key words: cotton fiber, fiber cell elongation, MYB–HD‐ZIPtranscription complex, phospholipid transport, transcriptionalregulation

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